Flow cytometric analysis of CD4 and T-lymphocytes of chronic amphetamine and alcohol abuse.

IF 0.8 4区医学 Q4 NEUROSCIENCES

Archives Italiennes De Biologie Pub Date : 2021-12-31 DOI:10.12871/000398292021342

K M Mehdar, A R Alrafiah

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引用次数: 0

Abstract

Introduction: Drug and alcohol abuse has significant negative impacts on individuals' life, health status and communities. They have also been implicated immunity system diseases. Therefore we aim to investigate the impact of chronic amphetamine abuse or co-abuse of alcohol with amphetamine on immunity, especially CD3 T cells, as well as the response of CD4 and CD8 T cells.

Patients and methods: A research sample comprising of 53 individuals younger than 40 years of age was employed to perform a retrospective case study within the setting of the Al-Amal Psychiatric Hospital. Individuals were distributed into 3 groups as the following: group (i) 17 patients abused amphetamine only for five years, group (ii) 17 patients' co-abused alcohol and amphetamine or five years, and group (iii) composed of 19 non-abusers of similar age and sex was selected as control. Flow cytometry was conducted for the collection and analysis of CD3+CD4+ and CD3+CD8+ monoclonal antibodies from blood samples.

Results: In contrast to the control group, amphetamine abuser patients showed a significantly decrease of the mean levels of CD3+CD4+ (#Event), CD3+CD8+ (#Event) and CD3+CD8+ (% Total). Meanwhile, levels of CD3+CD4+ (% Parent) was significantly higher in patients versus control (P = 0.001). furthermore, In the amphetamine abuse patient group, there were significant positive correlations between CD3+CD4+ (#Event) and CD3+CD4+ (% Total); CD3+CD8+ (#Event); Between CD3+CD4+ (% Parent) and CD3+CD8+ (% Parent) and CD3+CD8+ (% Total); Between CD3+CD4+ (% Total) and CD3+CD8+ (#Event); Between CD3+CD8+ % Parent) and CD3+CD8+ (% Total).

Conclusion: The present work shed light on the reaction of CD4 and CD8 T cells to chronic amphetamine and alcohol abuse, which was found to induce changes in the systemic immune response. Interestingly, amphetamine abuse markedly changes the systemic immune as they diminished CD3+CD4+ and CD3+CD8+ T cells, and amphetamine and co-abuse alcohol affected CD3 cells significantly.

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慢性苯丙胺和酒精滥用患者CD4和t淋巴细胞的流式细胞分析。

药物和酒精滥用对个人生活、健康状况和社区都有重大的负面影响。它们也与免疫系统疾病有关。因此，我们的目的是研究慢性滥用安非他明或同时滥用酒精和安非他明对免疫，特别是CD3 T细胞，以及CD4和CD8 T细胞反应的影响。患者和方法:在Al-Amal精神病医院进行回顾性病例研究，研究样本包括53名年龄小于40岁的人。将个体分为3组:组(i) 17例患者仅滥用安非他明5年，组(ii) 17例患者同时滥用酒精和安非他明5年，组(iii) 19例年龄和性别相似的非滥用者作为对照。采用流式细胞术采集和分析血样中CD3+CD4+和CD3+CD8+单克隆抗体。结果:与对照组相比，安非他命滥用者CD3+CD4+ (#Event)、CD3+CD8+ (#Event)和CD3+CD8+ (% Total)的平均水平均显著降低。同时，患者的CD3+CD4+ (% Parent)水平显著高于对照组(P = 0.001)。此外，在安非他明滥用患者组中，CD3+CD4+ (#Event)与CD3+CD4+ (% Total)之间存在显著正相关;CD3 + CD8 +(#事件);CD3+CD4+(%亲本)、CD3+CD8+(%亲本)和CD3+CD8+ (% Total)之间;CD3+CD4+ (% Total)和CD3+CD8+ (#Event)之间;介于CD3+CD8+ % Parent)和CD3+CD8+ (% Total)之间。结论:本研究揭示了CD4和CD8 T细胞对慢性苯丙胺和酒精滥用的反应，发现其可诱导全身免疫反应的变化。有趣的是，滥用安非他明显著改变全身免疫，因为它们减少了CD3+CD4+和CD3+CD8+ T细胞，安非他明和共滥用酒精显著影响CD3细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives Italiennes De Biologie 医学-神经科学

CiteScore

2.10

自引率

30.00%

发文量

审稿时长

>12 weeks

期刊介绍： Archives Italiennes de Biologie - a Journal of Neuroscience- was founded in 1882 and represents one of the oldest neuroscience journals in the world. Archives publishes original contributions in all the fields of neuroscience, including neurophysiology, experimental neuroanatomy and electron microscopy, neurobiology, neurochemistry, molecular biology, genetics, functional brain imaging and behavioral science. Archives Italiennes de Biologie also publishes monographic special issues that collect papers on a specific topic of interest in neuroscience as well as the proceedings of important scientific events. Archives Italiennes de Biologie is published in 4 issues per year and is indexed in the major collections of biomedical journals, including Medline, PubMed, Current Contents, Excerpta Medica.