Decreased diversity of salivary microbiome in patients with stable decompensated cirrhosis.

IF 0.3 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Hippokratia Pub Date : 2020-10-01

T Oikonomou, E Cholongitas, G Gioula, F Minti, A Melidou, E Protonotariou, E Akriviadis, I Goulis

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引用次数: 0

Abstract

Background: In the setting of the oral-gut-liver axis, microbiome dysbiosis has been associated with decompensated cirrhosis progression. However, little is known on salivary microbiome profiles in stable decompensated patients.

Methods: We studied patients with stable decompensated cirrhosis (n =28) and matched healthy controls (n =26). There were five patients (17.8 %) with hepatocellular carcinoma (HCC). Microbiomes of the 54 salivary samples were profiled through next-generation sequencing of the 16S-rRNA region in bacteria.

Results: The two study groups (patients and controls) did not differ significantly concerning their baseline characteristics. The most abundant phyla were Firmicutes, Bacteroidetes, Proteobacteria, and Fusobacteria. Proposed dysbiosis ratio Firmicutes/Bacteroidetes was lower in patients than in controls (range: 0.05-2.54 vs. 0.28-2.18, p =0.4), showing no statistical significance. Phylum Deinococcus-Thermus was detected only in controls, while Phylum Planctomycetes only in patients. A-diversity analysis indicated low diversity of salivary microbiome in decompensated patients and patients with HCC, who presented specific discriminative taxa. On principal coordinate analysis (PCoA), the patients' and controls' salivary microbiomes clustered apart, suggesting differences in community composition (PERMANOVA test, p =0.008). Boruta wrapper algorithm selected the most representative genera to classify controls and patients (area under the curve =0.815).

Conclusions: Patients with stable decompensated cirrhosis of various etiology and history of complications have decreased diversity of their salivary microbiome. PCoA and Boruta algorithm may represent useful tools to discriminate the salivary microbiome in patients with decompensation. Further studies are needed to establish the utility of salivary microbiome analysis, which is easier obtained than fecal, in decompensated cirrhosis. HIPPOKRATIA 2020, 24(4): 157-165.

本刊更多论文

稳定失代偿肝硬化患者唾液微生物群多样性降低。

背景:在口腔-肠-肝轴的情况下，微生物群失调与失代偿性肝硬化进展有关。然而，对稳定失代偿患者的唾液微生物组谱知之甚少。方法:我们研究了稳定失代偿性肝硬化患者(n =28)和匹配的健康对照(n =26)。肝细胞癌5例(17.8%)。通过细菌16S-rRNA区域的下一代测序，对54份唾液样本的微生物组进行了分析。结果:两个研究组(患者和对照组)的基线特征没有显著差异。最丰富的门是厚壁菌门、拟杆菌门、变形菌门和梭杆菌门。建议的菌群失调比例患者厚壁菌门/拟杆菌门低于对照组(范围:0.05-2.54比0.28-2.18,p =0.4)，差异无统计学意义。热球菌门仅在对照组中检测到，而植物门仅在患者中检测到。a -多样性分析表明，失代偿患者和HCC患者的唾液微生物组多样性较低，具有特异性的鉴别分类群。在主坐标分析(PCoA)中，患者和对照组的唾液微生物组聚在一起，表明群落组成存在差异(PERMANOVA检验，p =0.008)。Boruta包装算法选取最具代表性的属对对照组和患者进行分类(曲线下面积=0.815)。结论:各种病因和并发症史的稳定失代偿性肝硬化患者唾液微生物群多样性降低。PCoA和Boruta算法可能是鉴别失代偿患者唾液微生物组的有用工具。需要进一步的研究来确定唾液微生物组分析在失代偿肝硬化中的效用，这比粪便更容易获得。中华医学杂志，2020,24(4):157-165。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hippokratia MEDICINE, GENERAL & INTERNAL-

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Hippokratia journal is a quarterly issued, open access, peer reviewed, general medical journal, published in Thessaloniki, Greece. It is a forum for all medical specialties. The journal is published continuously since 1997, its official language is English and all submitted manuscripts undergo peer review by two independent reviewers, assigned by the Editor (double blinded review process). Hippokratia journal is managed by its Editorial Board and has an International Advisory Committee and over 500 expert Reviewers covering all medical specialties and additionally Technical Reviewers, Statisticians, Image processing Experts and a journal Secretary. The Society “Friends of Hippokratia Journal” has the financial management of both the printed and electronic edition of the journal.