VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting Halorubrum.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2021-12-14 eCollection Date: 2021-01-01 DOI:10.1155/2021/8271899

Shaoxing Chen, Yongpei Dai, Jingwen Liu, Shimin Zhang, Feilong Chen, Fanjie Jin, Peiyao Ruan, Lu Li, Xiangdong Chen

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引用次数: 3

Abstract

A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon Halorubrum sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of Halorubrum sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of Halorubrum sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (w/v). VOLN27B and Halorubrum sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.

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从地下盐晶体中分离出一种新的头尾盐病毒并感染盐丛。

在白垩纪时期(中国安徽)形成的地层中，从深度约430 m的岩心样本中分离出一种新的halovirus, VOLN27B。VOLN27B感染嗜盐古菌Halorubrum sp. LN27，具有典型的肌病毒的头尾形态，尾部可收缩。平均头径64±2.0 nm，未收缩尾径15±1.0 × 65±2.0 nm。潜伏期约10小时。VOLN27B在Halorubrum sp. LN27细胞中的成熟时间接近8 h。VOLN27B在Halorubrum sp. LN27细胞上的吸附时间小于1 min。当pH值小于5或NaCl浓度低于12% (w/v)时，病毒颗粒不稳定。VOLN27B和Halorubrum sp. LN27是从相同的高盐环境中分离出来的，在盐古菌中提供了一种新的病毒宿主系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464