Research on the mechanisms of taraxerol for the treatment of gastric cancer effect based on network pharmacology.

IF 3 3区医学 Q3 IMMUNOLOGY

International Journal of Immunopathology and Pharmacology Pub Date : 2022-01-01 DOI:10.1177/20587384211063962

Bingjie Huo, Yanru Song, Bibo Tan, Jianbo Li, Jie Zhang, Fengbin Zhang, Liang Chang

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引用次数: 1

Abstract

Background: Modern pharmacological studies have shown that traditional Chinese medicine (TCM) Taraxacum mongolicum possesses anti-cancer activity. Taraxerol (TRX) is a pentacyclic triterpene isolated from T. mongolicum, which is widely used in clinical treatment, and its anti-cancer effects have been extensively studied. However, the effects and molecular mechanism of TRX in gastric cancer (GC) have not been fully explicated.

Methods: We used public databases to derive information on potential targets of TRX and proteins related to GC. Also, STRING and R3.6.2 software were used to analyze the protein-protein interaction (PPI). The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were done to explain the potential mechanism underlying the regulatory role of TRX in GC. The role of TRX in GC was verified by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, apoptosis analysis, Transwell assay, and wound healing assay, and the key signaling pathways were verified.

Results: We identified 135 potential targets for the treatment of GC via network pharmacological analysis. GO and KEGG enrichment analysis showed that steroid hormone receptor activity and the PI3K/AKT signaling pathway were the biological processes and pathways with the highest degree of enrichment. Additionally, cellular experiments revealed that TRX inhibited the proliferation, migration, and invasion of GC cells as well as induced G1 phase arrest and apoptosis in GC cells.

Conclusion: Here, we used multi-target and multi-pathway network pharmacological analysis to verify the anti-cancer activity of TRX in GC. Also, in vitro experimental data were used to derive the potential molecular mechanism.

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基于网络药理学的taraxerol治疗胃癌作用机制研究。

背景:现代药理研究表明，中药蒲公英具有抗癌活性。Taraxerol (TRX)是一种从蒙古金霉中分离得到的五环三萜，广泛应用于临床治疗，其抗癌作用已被广泛研究。然而，TRX在胃癌(GC)中的作用和分子机制尚未完全阐明。方法:利用公共数据库获取TRX潜在靶点和GC相关蛋白的信息。采用STRING和R3.6.2软件分析蛋白-蛋白相互作用(PPI)。通过基因本体(GO)和京都基因与基因组百科全书(KEGG)分析来解释TRX在GC中调控作用的潜在机制。通过3-(4,5)-二甲基噻吩偶氮(-z-y1)-3,5-二-苯四唑脲(MTT)实验、细胞凋亡分析、Transwell实验和伤口愈合实验验证了TRX在GC中的作用，并验证了关键信号通路。结果:通过网络药理分析，我们确定了135个治疗GC的潜在靶点。GO和KEGG富集分析显示，类固醇激素受体活性和PI3K/AKT信号通路是富集程度最高的生物学过程和途径。此外，细胞实验显示，TRX抑制GC细胞的增殖、迁移和侵袭，并诱导GC细胞G1期阻滞和凋亡。结论:本研究通过多靶点、多通路网络药理分析，验证了TRX在胃癌中的抗癌作用。并利用体外实验数据推导了其潜在的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Immunopathology and Pharmacology 医学-病理学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.