{"title":"Experimental Autoimmune Encephalomyelitis in the Mouse.","authors":"Collin Laaker, Martin Hsu, Zsuzsanna Fabry, Stephen D Miller, William J Karpus","doi":"10.1002/cpz1.300","DOIUrl":null,"url":null,"abstract":"<p><p>This article details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Additionally, active induction of EAE in the C57BL/6 strain using myelin oligodendrocyte glycoprotein (MOG) peptide is also discussed. Detailed materials and methods required for the purification of both PLP and MBP are described, and a protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains. © 2021 Wiley Periodicals LLC. Basic Protocol: Active induction of EAE with PLP, MBP, and MOG protein or peptide Alternate Protocol: Adoptive induction of EAE with PLP-, MBP-, or MOG-specific lymphocytes Support Protocol 1: Purification of proteolipid protein Support Protocol 2: Purification of myelin basic protein Support Protocol 3: Isolation of CNS-infiltrating lymphocytes.</p>","PeriodicalId":11174,"journal":{"name":"Current Protocols","volume":" ","pages":"e300"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/cpz1.300","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This article details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Additionally, active induction of EAE in the C57BL/6 strain using myelin oligodendrocyte glycoprotein (MOG) peptide is also discussed. Detailed materials and methods required for the purification of both PLP and MBP are described, and a protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains. © 2021 Wiley Periodicals LLC. Basic Protocol: Active induction of EAE with PLP, MBP, and MOG protein or peptide Alternate Protocol: Adoptive induction of EAE with PLP-, MBP-, or MOG-specific lymphocytes Support Protocol 1: Purification of proteolipid protein Support Protocol 2: Purification of myelin basic protein Support Protocol 3: Isolation of CNS-infiltrating lymphocytes.
小鼠实验性自身免疫性脑脊髓炎
本文详细介绍了使用完整蛋白或两种主要髓鞘蛋白（蛋白脂蛋白（PLP）和髓鞘碱性蛋白（MBP））的肽对SJL小鼠品系进行实验性自身免疫性脑脊髓炎（EAE）的主动诱导和收养性转移所需的材料和方法。此外，还讨论了使用髓鞘少突胶质细胞糖蛋白（MOG）肽主动诱导 C57BL/6 株 EAE 的问题。此外，还介绍了纯化 PLP 和 MBP 所需的详细材料和方法，以及在 EAE 小鼠中分离中枢神经系统浸润淋巴细胞的方案。要在其他小鼠品系中有效诱导活动性或收养性 EAE，可能需要对指定方案进行修改。© 2021 Wiley Periodicals LLC.基本方案：用 PLP、MBP 和 MOG 蛋白或肽主动诱导 EAE 备用方案：支持方案 1：纯化蛋白脂蛋白 支持方案 2：纯化髓鞘碱性蛋白 支持方案 3：分离中枢神经系统浸润淋巴细胞。
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