Evaluation of Pleotropic Protective Activity of Capparis spinose Extract on Arthritis Rat Model.

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2022-01-01 DOI:10.2174/1871523021666220211110136

Mohammed Yosri, Mahmoud M Elaasser, Marwa M Abdel-Aziz, Hanna Y Ahmed, Basma H Amin

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引用次数: 2

Abstract

Background: Capparis spinosa grows in Asian and Mediterranean desert areas. Different parts of Capparis spinosa, including flowers, have been used in various folk medicine applications.

Objective: This study aims to evaluate the anti-arthritic potential of ethanolic extract of Egyptian Capparis spinosa flowers in a rat model of rheumatoid arthritis. Moreover, analysis of Capparis spinosa extract was performed using LC-qTOF-MS/MS.

Methods: Animals were split into six groups: negative control group, induced arthritic animals, arthritic rats receiving 7, 14 and 28 mg/kg of Capparis spinosa extract, respectively, in three groups to detect the optimum dose, and the induced group receiving a standard drug. The arthritic score was checked daily for 15 days after induction. After animals were sacrificed, their joints and muscles were subjected to microscopic and ultra-structure examinations. Ex vivo culturing of osteoclasts was performed. Cytokine levels were measured in all examined groups.

Results: The results revealed 7 mg/kg of Capparis spinosa extract as the optimal dose, which decreased inflammation signs through controlling chondrocytes, osteoclasts, and levels of inflammatory mediators.

Conclusion: LC-Mass analysis revealed Capparis spinosa extract to contain a mixture of flavonol glycosides, flavan-3-ols and hydroxycinnamic acid derivatives, which may provide beneficial multifunction in regulating arthritic symptoms.

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刺槐提取物对关节炎大鼠模型的多效性保护作用评价。

背景:刺蒺藜生长在亚洲和地中海沙漠地区。刺蒺藜的不同部分，包括花，已被用于各种民间医学应用。目的:研究埃及刺荆花乙醇提取物对类风湿关节炎大鼠模型的抗关节炎作用。此外，采用LC-qTOF-MS/MS对刺毛柑提取物进行分析。方法:将动物分为6组:阴性对照组、诱导性关节炎动物、关节炎大鼠分别给予7、14、28 mg/kg刺荆芥提取物，三组检测最佳剂量，诱导组给予标准药物。诱导后15天每天检查关节炎评分。动物处死后，对关节和肌肉进行显微和超微结构检查。体外培养破骨细胞。检测各组细胞因子水平。结果:以7 mg/kg刺毛猴提取物为最佳剂量，通过控制软骨细胞、破骨细胞和炎症介质水平来降低炎症体征。结论:液相色谱-质谱分析表明，香椒提取物含有黄酮醇苷、黄烷-3-醇和羟基肉桂酸衍生物，可能具有多种调节关节炎症状的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.