Effects of betulinic acid on AKT/mTOR pathway in renal cell carcinoma.

IF 1 Q4 UROLOGY & NEPHROLOGY
Merve Nur Ataş, Barış Ertuğrul, Elif Sinem İplik, Bedia Çakmakoğlu, Arzu Ergen
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引用次数: 0

Abstract

Objective: Renal cancer is the most lethal among urological cancer. Treatments of renal cell carcinoma (RCC) may be possible by immune checkpoint inhibitors and drug treatment targeting different molecules. We aimed to determine the apoptotic effect of betulinic acid and its effects on expressions of apoptosisassociated genes AKT-1 and mTOR in RCC cells.

Material and methods: In this study, we investigated the apoptotic activity of betulinic acid in CAKI-2 cell line and its effect on AKT-1 and mTOR gene expression levels. In order to do so, following analyses were conducted: WST-1 to identify the toxic effect of betulinic acid, Caspase-3/BCA to detect caspase enzyme activity, Annexin-V and ELISA to determine for apoptotic effect, and finally, real-time PCR for expression levels of AKT-1 and mTOR.

Results: Our study showed that different concentrations of betulinic acid induced apoptosis in renal cancer; however, no effect was observed in healthy cells. In gene expression analysis, there was statistically significant decrease in AKT-1 expression level while increasing mTOR expression level.

Conclusion: We suggested that betulinic acid with its apoptotic effect on RCC line and nontoxic effect on healthy cell line and the effects on AKT/mTOR pathway may be a potential anticancer drug promising for future studies.

Abstract Image

Abstract Image

白桦酸对肾细胞癌AKT/mTOR通路的影响。
目的:肾癌是泌尿系统肿瘤中致死率最高的。通过免疫检查点抑制剂和针对不同分子的药物治疗,肾细胞癌(RCC)的治疗可能成为可能。我们旨在研究白桦酸对RCC细胞的凋亡作用及其对凋亡相关基因AKT-1和mTOR表达的影响。材料与方法:本实验研究白桦酸对CAKI-2细胞株的凋亡活性及其对AKT-1和mTOR基因表达水平的影响。为此,我们进行了以下分析:WST-1检测白桦酸的毒性作用,caspase -3/BCA检测caspase酶活性,Annexin-V和ELISA检测凋亡作用,最后real-time PCR检测AKT-1和mTOR的表达水平。结果:我们的研究表明,不同浓度的白桦酸可诱导肾癌细胞凋亡;然而,在健康细胞中没有观察到任何影响。基因表达分析中,AKT-1表达水平降低,mTOR表达水平升高,差异有统计学意义。结论:白桦酸对RCC细胞的凋亡作用和对健康细胞的无毒作用以及对AKT/mTOR通路的影响可能是一种潜在的抗癌药物,值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Turkish journal of urology
Turkish journal of urology Medicine-Urology
CiteScore
2.10
自引率
0.00%
发文量
53
期刊介绍: The aim of the Turkish Journal of Urology is to contribute to the literature by publishing scientifically high-quality research articles as well as reviews, editorials, letters to the editor and case reports. The journal’s target audience includes, urology specialists, medical specialty fellows and other specialists and practitioners who are interested in the field of urology.
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