Level of Pyrethroid-Resistance Associated with Cytochrome P450 Expression in German Cockroach Blattella germanica (Blattodea: Ectobiidae) in the Field Collected Strains.

IF 0.6 4区医学 Q4 PARASITOLOGY

Journal of Arthropod-Borne Diseases Pub Date : 2021-06-30 eCollection Date: 2021-06-01 DOI:10.18502/jad.v15i2.7484

Azimeh Ghaderi, Mozghan Baniardalani, Hamid Reza Basseri

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引用次数: 1

Abstract

Background: Cytochrome P450-dependent monooxygenases are a very important metabolic system involve in insecticide resistance. This study was conducted to find the association between the expression level of cytochrome P450 (CYP450) and permethrin-resistance level among four strains of the German cockroach Blattella germanica (L) (Blattodea: Ectobiidae).

Methods: Three field strains of German cockroach with different frequency of exposure to pesticides, and a laboratory susceptible strain were used in the present study. Insecticide susceptibility bioassays were carried out to detect resistance to permethrin. The concentration of CYP450 in each strain was determined using ion-exchange HPLC chromatography. Biochemical assays was performed to analyse CYP450 activities.

Results: The resistance ratios (RR) to permethrin among three field strains were 3.29, 4.10 and 6.17-fold comping with the susceptible stain. The CYP450 activity of three field strains was 1.6, 2.4 and 2.7 times higher than in the susceptible stain. The amount of CYP450 per mg of protein was significantly different between the susceptible and the three resistant strains. The resistant cockroaches showed a relatively high expression of CYP450 enzymes. A strong correlation was found between permethrin resistance level and total concentration of CYP450 enzymes.

Conclusion: The results of current study show that more frequent usage of a pyrethroid insecticide cause the metabolic insecticide resistance to rise in German cockroach. Therefore, there is a ratio correlation between resistance level and monooxygenases activities in insect. Thus, the control program must be managed according to history of pesticide usage.

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田间收集品系德国小蠊抗拟除虫菊酯水平与细胞色素P450表达的关系

背景:细胞色素p450依赖性单加氧酶是参与杀虫剂抗性的重要代谢系统。本研究旨在研究4种德国小蠊(Blattella germanica, L)细胞色素P450 (CYP450)表达水平与氯菊酯抗性水平的关系。方法:采用3株不同农药暴露频率的田间德国蜚蠊和1株实验室敏感品系进行研究。采用药敏生物试验检测对氯菊酯的抗性。采用离子交换高效液相色谱法测定各菌株中CYP450的浓度。生化检测CYP450活性。结果:3个田间品系对氯菊酯的抗性分别为敏感品系的3.29倍、4.10倍和6.17倍。田间菌株CYP450活性分别比敏感菌株高1.6倍、2.4倍和2.7倍。CYP450 / mg蛋白含量在3个耐药菌株和敏感菌株之间存在显著差异。抗性蟑螂CYP450酶的表达量较高。氯菊酯抗性水平与CYP450酶总浓度有较强的相关性。结论:目前的研究结果表明，使用拟除虫菊酯类杀虫剂的频率增加会导致德国蜚蠊代谢性抗药性上升。因此，昆虫的抗性水平与单加氧酶活性之间存在一定的比例相关性。因此，控制程序必须根据农药使用历史进行管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

8 weeks

期刊介绍： The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.