Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2022-01-07 eCollection Date: 2022-01-01 DOI:10.1155/2022/8560831

Xiaohua Duan, Jianlin Lv, Hebei Jiang, Kefei Zheng, Yulin Chen

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引用次数: 3

Abstract

Objectives: The occurrence and development of nonalcoholic fatty liver disease (NAFLD) is related to lipid peroxidation, imbalance of inflammatory response factors, and immune function disorder. This study was conducted with the purpose of investigating the expression levels of inflammatory cytokines and adipocytokines and Th17/Treg balance in NAFLD patients treated with Dahuang Zhechong pills (DHZCPs).

Methods: The study recruited 100 NAFLD patients who were then arranged into the test group and control group. Patients in the test group were treated with DHZCPs, while patients in the control group were untreated. Peripheral TH17 and Treg cells were detected by flow cytometry, and peripheral IL-17, IL-10, hs-CRP, and TNF-α expression levels were determined by enzyme-linked immunosorbent assay (ELISA) methods. The concentrations of ghrelin, leptin, and adiponectin were quantitatively examined.

Results: The levels of TC, TG, ALT, and AST were declined but the level of HDL-C was increased in NAFLD patients treated with DHZCPs compared with untreated patients (P < 0.05). The ratio of Th17/Treg in NAFLD patients treated with DHZCPs was (1.52 ± 0.21), which was significantly lower than (2.39 ± 0.45) of untreated patients (P < 0.05). The levels of IL-17, hs-CRP, and TNF-α were lower, but the level of IL-10 was higher in NAFLD patients treated with DHZCPs than that in untreated patients (P < 0.05). The expression levels of ghrelin and adiponectin in NAFLD patients treated with DHZCPs were evidently higher than those in untreated patients (P < 0.01), and the expression level of leptin in NAFLD patients treated with DHZCPs was evidently lower than that in untreated patients (P < 0.01).

Conclusions: Administration of DHZCPs regulates the immune function of NAFLD patients by keeping Th17/Treg balance and affecting the levels of inflammatory cytokines and adipocytokines.

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非酒精性脂肪肝患者服用大黄降脂丸后炎症因子、脂肪因子和Th17/Treg平衡的变化

目的:非酒精性脂肪性肝病(NAFLD)的发生发展与脂质过氧化、炎症反应因子失衡、免疫功能紊乱有关。本研究旨在探讨大黄降脂丸(DHZCPs)对NAFLD患者炎症因子和脂肪因子表达水平及Th17/Treg平衡的影响。方法:本研究招募100例NAFLD患者，将其分为试验组和对照组。试验组患者给予DHZCPs治疗，对照组患者不予治疗。采用流式细胞术检测外周血TH17和Treg细胞，采用酶联免疫吸附试验(ELISA)检测外周血IL-17、IL-10、hs-CRP和TNF-α表达水平。定量检测胃饥饿素、瘦素和脂联素的浓度。结果:与未治疗组相比，经DHZCPs治疗的NAFLD患者TC、TG、ALT、AST水平下降，HDL-C水平升高(P < 0.05)。DHZCPs组患者Th17/Treg比值为(1.52±0.21)，显著低于未治疗组(2.39±0.45)，差异有统计学意义(P < 0.05)。治疗组IL-17、hs-CRP、TNF-α水平低于对照组，IL-10水平高于对照组(P < 0.05)。DHZCPs治疗组胃饥饿素和脂联素表达水平明显高于未治疗组(P < 0.01)，瘦素表达水平明显低于未治疗组(P < 0.01)。结论:DHZCPs通过维持Th17/Treg平衡，影响炎症因子和脂肪因子水平，调节NAFLD患者的免疫功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.