SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells.

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI:10.1387/ijdb.210062zz

Rui-Fang Li, Guo-Xin Nan, Dan Wang, Chang Gao, Juan Yang, Tong-Chuan He, Zhong-Lin Zhang

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引用次数: 0

Abstract

Background: The specific effect of SV40T on neurocytes has seldom been investigated by the researchers. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T, in order to explore the effects of SV40T on Schwann cells.

Methods: SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, and also co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry.

Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus. In addition, SV40T up-regulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells.

Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells (NCCs) that can differentiate to neurocytes.

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SV40T将雪旺细胞重编程为干细胞样细胞，可以重新分化为末梢神经细胞。

背景:SV40T对神经细胞的特异性作用研究很少。我们用含有SV40T的MPH 86质粒转染不具有分化能力的雪旺细胞(SCs)，探讨SV40T对雪旺细胞的影响。方法:用携带SV40T基因的MPH 86质粒转染SCs，在不同培养基中培养，并与神经干细胞(NSCs)共培养。在我们的研究中，过表达SV40T的SCs被定义为SV40T-SCs。WST-1检测各组细胞的增殖情况，qPCR和免疫组织化学检测各组细胞中不同生物标志物的表达情况。结果:SV40T诱导的NSCs具有悬浮生长、形成球形菌落、快速增殖等特性，而flip腺病毒敲除SV40T后，这些特性被逆转。此外，SV40T上调神经嵴相关标志物Nestin、Pax3和Slug的表达，下调S100b以及成熟SCs标志物MBP、GFAP和Olig1/2的表达。这些细胞还表达Nestin、Sox2、CD133、SSEA-1等NSC标记物，以及BMP4、c-Myc、OCT4、Gbx2等胚胎干细胞早期发育标记物。与NSCs共培养可诱导SV40T-SCs分化为神经元细胞和胶质细胞。结论:SV40T在神经嵴细胞(NCCs)阶段将雪旺细胞重编程为可分化为神经细胞的干细胞样细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Developmental Biology 生物-发育生物学

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The International Journal of Developmental Biology (ISSN: 0214- 6282) is an independent, not for profit scholarly journal, published by scientists, for scientists. The journal publishes papers which throw light on our understanding of animal and plant developmental mechanisms in health and disease and, in particular, research which elucidates the developmental principles underlying stem cell properties and cancer. Technical, historical or theoretical approaches also fall within the scope of the journal. Criteria for acceptance include scientific excellence, novelty and quality of presentation of data and illustrations. Advantages of publishing in the journal include: rapid publication; free unlimited color reproduction; no page charges; free publication of online supplementary material; free publication of audio files (MP3 type); one-to-one personalized attention at all stages during the editorial process. An easy online submission facility and an open online access option, by means of which papers can be published without any access restrictions. In keeping with its mission, the journal offers free online subscriptions to academic institutions in developing countries.