Genetic Diversity and Population Structure of Moroccan Isolates Belong to Alternaria spp. Causing Black Rot and Brown Spot in Citrus.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
International Journal of Genomics Pub Date : 2021-11-23 eCollection Date: 2021-01-01 DOI:10.1155/2021/9976969
Lamyaa Zelmat, Joseph Mbasani Mansi, Sarra Aouzal, Fatima Gaboun, Slimane Khayi, Mohammed Ibriz, Mohammed El Guilli, Rachid Mentag
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引用次数: 6

Abstract

Alternaria alternata is one of the most important fungi causing various diseases on citrus worldwide. In Morocco, Alternaria black rot (ABR) and Alternaria brown spot (ABS) are two major diseases causing serious losses in commercial cultivars of citrus. The aim of the present work was to study the genetic diversity and the population structure of isolates belonging to sect. Alternaria obtained from infected citrus fruits, collected from seven provinces at different locations in Morocco (markets, packinghouses, and orchards). Forty-five isolates were analyzed by sequence-related amplified polymorphism (SRAP) markers, and cluster analysis of DNA fragments was performed using UPGMA method and Jaccard coefficient. Cluster analysis revealed that isolates were classified in four distinct groups. AMOVA revealed also a large extent of variation within sect. Alternaria isolates (99%). The results demonstrate that no correlation was found among SRAP pattern, host, and geographical origin of these isolates. Population structure analyses showed that the Alternaria isolates from the same collection origin had almost a similar level of admixture.

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柑橘黑腐病和褐斑病摩洛哥分离株的遗传多样性和群体结构。
Alternaria alternata是世界范围内引起柑橘多种疾病的重要真菌之一。在摩洛哥,链格孢黑腐病(ABR)和链格孢褐色斑点病(ABS)是造成柑橘商业品种严重损失的两种主要病害。本研究的目的是研究该菌株的遗传多样性和种群结构。链格孢菌是从摩洛哥七个省不同地点(市场、包装厂和果园)采集的受感染柑橘类水果中获得的。用序列相关扩增多态性(SRAP)标记对45个分离株进行分析,并用UPGMA法和Jaccard系数对DNA片段进行聚类分析。聚类分析显示,分离物分为四个不同的组。AMOVA还揭示了教派内部的很大程度的差异。链格孢分离株(99%)。结果表明,这些分离株的SRAP模式、宿主和地理来源之间没有相关性。种群结构分析表明,来自同一采集来源的链格孢分离株具有几乎相似的混合水平。
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来源期刊
International Journal of Genomics
International Journal of Genomics BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CiteScore
5.40
自引率
0.00%
发文量
33
审稿时长
17 weeks
期刊介绍: International Journal of Genomics is a peer-reviewed, Open Access journal that publishes research articles as well as review articles in all areas of genome-scale analysis. Topics covered by the journal include, but are not limited to: bioinformatics, clinical genomics, disease genomics, epigenomics, evolutionary genomics, functional genomics, genome engineering, and synthetic genomics.
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