Effect of a copper intrauterine device on HLA-G and IGF-II levels during pregnancy

IF 1.6 4区医学 Q4 CELL BIOLOGY

Growth Hormone & Igf Research Pub Date : 2022-02-01 DOI:10.1016/j.ghir.2021.101441

Lili Cao , Xiuying Chen , Lili Huang

{"title":"Effect of a copper intrauterine device on HLA-G and IGF-II levels during pregnancy","authors":"Lili Cao , Xiuying Chen , Lili Huang","doi":"10.1016/j.ghir.2021.101441","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>An intrauterine device (IUD) is one of the most effective reversible contraceptive methods currently available. Women who use IUDs may become pregnant, albeit rarely, and many such women continue to use IUDs. Because it is difficult to remove or it may cause miscarriage. This study measured the changes in human leucocyte antigen-G (HLA-G) and insulin-like growth factor II (IGF-II) levels in the decidua and villi to explore the effect of a copper IUD on embryonic development.</p></div><div><h3>Design</h3><p>A total of 54 samples of decidual and villus tissue were collected from pregnant women with IUDs (27 samples) or without IUDs (27 samples). Hematoxylin-eosin staining was used to identify morphological characteristics. Immunohistochemistry was used to detect HLA-G and IGF-II; the protein expression levels were measured via Western blotting.</p></div><div><h3>Results</h3><p>HLA-G was expressed on the membranes of trophoblasts of villus tissues and the glandular epithelium, and in stromal cells of decidual tissues, in both the IUD and control groups. IGF-II was expressed in the glandular epithelium and cytoplasm of trophoblasts and decidual cells in both groups. Compared to the control group, IGF-II expression was significantly reduced in villus tissues of the IUD group (p < 0.05). The mean sac diameter was significantly positively correlated with IGF-II expression in the villi (<em>p</em> < 0.05).</p></div><div><h3>Conclusions</h3><p>A copper IUD may affect embryonic development by regulating the expression of villus IGF-II.</p></div>","PeriodicalId":12803,"journal":{"name":"Growth Hormone & Igf Research","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1096637421000642/pdfft?md5=1e8c04839a2ab881e9049c5a50c93b00&pid=1-s2.0-S1096637421000642-main.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Growth Hormone & Igf Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1096637421000642","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

Objective

An intrauterine device (IUD) is one of the most effective reversible contraceptive methods currently available. Women who use IUDs may become pregnant, albeit rarely, and many such women continue to use IUDs. Because it is difficult to remove or it may cause miscarriage. This study measured the changes in human leucocyte antigen-G (HLA-G) and insulin-like growth factor II (IGF-II) levels in the decidua and villi to explore the effect of a copper IUD on embryonic development.

Design

A total of 54 samples of decidual and villus tissue were collected from pregnant women with IUDs (27 samples) or without IUDs (27 samples). Hematoxylin-eosin staining was used to identify morphological characteristics. Immunohistochemistry was used to detect HLA-G and IGF-II; the protein expression levels were measured via Western blotting.

Results

HLA-G was expressed on the membranes of trophoblasts of villus tissues and the glandular epithelium, and in stromal cells of decidual tissues, in both the IUD and control groups. IGF-II was expressed in the glandular epithelium and cytoplasm of trophoblasts and decidual cells in both groups. Compared to the control group, IGF-II expression was significantly reduced in villus tissues of the IUD group (p < 0.05). The mean sac diameter was significantly positively correlated with IGF-II expression in the villi (p < 0.05).

Conclusions

A copper IUD may affect embryonic development by regulating the expression of villus IGF-II.

查看原文本刊更多论文

铜宫内节育器对妊娠期HLA-G和IGF-II水平的影响

目的宫内节育器(IUD)是目前最有效的可逆避孕方法之一。使用宫内节育器的妇女可能会怀孕，尽管很少，而且许多这样的妇女继续使用宫内节育器。因为它很难取出或可能导致流产。本研究通过测定蜕膜和绒毛中人白细胞抗原g (HLA-G)和胰岛素样生长因子II (IGF-II)水平的变化，探讨铜宫内节育器对胚胎发育的影响。设计共收集有宫内节育器孕妇(27例)和无宫内节育器孕妇(27例)的蜕膜和绒毛组织54份。苏木精-伊红染色鉴定其形态特征。免疫组化检测HLA-G、IGF-II;Western blotting检测蛋白表达水平。结果在节育器组和对照组中，shla - g在绒毛组织、腺上皮滋养细胞膜和蜕膜间质细胞上均有表达。IGF-II在两组的腺上皮及滋养层细胞和蜕细胞的细胞质中均有表达。与对照组相比，IUD组绒毛组织中IGF-II表达显著降低(p <0.05)。平均囊直径与绒毛中IGF-II的表达呈显著正相关(p <0.05)。结论铜宫内节育器可能通过调节绒毛IGF-II的表达影响胚胎发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Growth Hormone & Igf Research 医学-内分泌学与代谢

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

57 days

期刊介绍： Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.