Classification and Morphology of Rhinocypha spp. (Odonata): A Comprehensive Taxonomic Study Within the Females.

IF 1.5 3区 生物学 Q2 ZOOLOGY
Zoological Studies Pub Date : 2021-08-26 eCollection Date: 2021-01-01 DOI:10.6620/ZS.2021.60-47
Noorhidayah Mamat, Arpah Abu, Norma-Rashid Yusoff
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引用次数: 1

Abstract

Studies on Odonata have gained attention worldwide as well as locally in Malaysia. Although there is a wealth of data available to be utilized for solving taxonomic problems, ecological and behavioural research areas are more favoured than taxonomy and systematics. Thus, there are confusions over how to correctly identify closely related and sympatric species, especially in female odonates. One such example is in the genus Rhinocypha. Consequently, the present study focuses on taxonomic work, employing multi-approaches in the form of morphological (morphological diagnostics, Field Emission Scanning Electron Microscope (FESEM) and geometric morphometric analysis), applying the molecular technique. Seventeen morphological characteristics were created to differentiate between the females of Rhinocypha spp. A FESEM was used on the female's ovipositor to focus on the anal appendages and sheathing valve (V3). Also, the phylogenetic patterns expressed by COI and 16S rRNA genes, and canonical variate analysis for the wing geometric morphometric revealed three clusters that supported the distinction of the Rhinocypha group. In summary, this study effectively developed an integrated approach of classic morphological and trendy molecular, combined with FESEM microscopy techniques, which provided corroborative evidence and resolved taxonomic uncertainties.

Abstract Image

鼻菇属的分类和形态:雌性内的综合分类研究。
对蛇腹虫的研究不仅在马来西亚引起了关注,而且在世界范围内也引起了关注。虽然有大量的数据可用于解决分类学问题,但生态和行为研究领域比分类学和系统学更受青睐。因此,对于如何正确识别近亲和同域物种,特别是雌性齿形动物,存在困惑。其中一个例子就是鼻菇属。因此,本研究的重点是分类工作,采用形态学(形态学诊断,场发射扫描电镜(FESEM)和几何形态计量学分析)形式的多种方法,应用分子技术。创建了17个形态学特征来区分鼻菇属雌虫。在雌性产卵器上使用FESEM聚焦于肛门附属物和鞘瓣(V3)。此外,COI和16S rRNA基因表达的系统发育模式以及对翅膀几何形态的典型变量分析显示,有3个簇支持了Rhinocypha类群的区分。综上所述,本研究有效地建立了经典形态学和新潮分子相结合的方法,并结合FESEM显微镜技术,提供了确凿的证据,解决了分类学的不确定性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Zoological Studies
Zoological Studies 生物-动物学
CiteScore
2.80
自引率
6.20%
发文量
35
审稿时长
6-12 weeks
期刊介绍: Zoological Studies publishes original research papers in five major fields: Animal Behavior, Comparative Physiology, Evolution, Ecology, and Systematics and Biogeography. Manuscripts are welcome from around the world and must be written in English. When the manuscript concerns the use of animals or specimens in research, a statement must be included to the effect that the author(s) has adhered to the legal requirements of the country in which the work was carried out or to any institutional guidelines.
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