{"title":"Classification and Morphology of <i>Rhinocypha</i> spp. (Odonata): A Comprehensive Taxonomic Study Within the Females.","authors":"Noorhidayah Mamat, Arpah Abu, Norma-Rashid Yusoff","doi":"10.6620/ZS.2021.60-47","DOIUrl":null,"url":null,"abstract":"<p><p>Studies on Odonata have gained attention worldwide as well as locally in Malaysia. Although there is a wealth of data available to be utilized for solving taxonomic problems, ecological and behavioural research areas are more favoured than taxonomy and systematics. Thus, there are confusions over how to correctly identify closely related and sympatric species, especially in female odonates. One such example is in the genus <i>Rhinocypha</i>. Consequently, the present study focuses on taxonomic work, employing multi-approaches in the form of morphological (morphological diagnostics, Field Emission Scanning Electron Microscope (FESEM) and geometric morphometric analysis), applying the molecular technique. Seventeen morphological characteristics were created to differentiate between the females of <i>Rhinocypha</i> spp. A FESEM was used on the female's ovipositor to focus on the anal appendages and sheathing valve (V3). Also, the phylogenetic patterns expressed by <i>COI</i> and 16S rRNA genes, and canonical variate analysis for the wing geometric morphometric revealed three clusters that supported the distinction of the <i>Rhinocypha</i> group. In summary, this study effectively developed an integrated approach of classic morphological and trendy molecular, combined with FESEM microscopy techniques, which provided corroborative evidence and resolved taxonomic uncertainties.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"60 ","pages":"e47"},"PeriodicalIF":1.5000,"publicationDate":"2021-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685346/pdf/zoolstud-60-047.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoological Studies","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.6620/ZS.2021.60-47","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
Studies on Odonata have gained attention worldwide as well as locally in Malaysia. Although there is a wealth of data available to be utilized for solving taxonomic problems, ecological and behavioural research areas are more favoured than taxonomy and systematics. Thus, there are confusions over how to correctly identify closely related and sympatric species, especially in female odonates. One such example is in the genus Rhinocypha. Consequently, the present study focuses on taxonomic work, employing multi-approaches in the form of morphological (morphological diagnostics, Field Emission Scanning Electron Microscope (FESEM) and geometric morphometric analysis), applying the molecular technique. Seventeen morphological characteristics were created to differentiate between the females of Rhinocypha spp. A FESEM was used on the female's ovipositor to focus on the anal appendages and sheathing valve (V3). Also, the phylogenetic patterns expressed by COI and 16S rRNA genes, and canonical variate analysis for the wing geometric morphometric revealed three clusters that supported the distinction of the Rhinocypha group. In summary, this study effectively developed an integrated approach of classic morphological and trendy molecular, combined with FESEM microscopy techniques, which provided corroborative evidence and resolved taxonomic uncertainties.
期刊介绍:
Zoological Studies publishes original research papers in five major fields: Animal Behavior, Comparative Physiology, Evolution, Ecology, and Systematics and Biogeography. Manuscripts are welcome from around the world and must be written in English. When the manuscript concerns the use of animals or specimens in research, a statement must be included to the effect that the author(s) has adhered to the legal requirements of the country in which the work was carried out or to any institutional guidelines.