Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Genomics Pub Date : 2021-11-15 eCollection Date: 2021-01-01 DOI:10.1155/2021/3752871

Zi-Qian Liang, Li Gao, Jun-Hong Chen, Wen-Bin Dai, Ya-Si Su, Gang Chen

{"title":"Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study.","authors":"Zi-Qian Liang, Li Gao, Jun-Hong Chen, Wen-Bin Dai, Ya-Si Su, Gang Chen","doi":"10.1155/2021/3752871","DOIUrl":null,"url":null,"abstract":"Introduction: We aimed to explore the downregulation of the coiled-coil domain containing 80 (CCDC80) and its underlying molecular mechanisms in ovarian carcinoma (OVCA). Materials/Methods. Immunohistochemical staining was performed to confirm the expression status of CCDC80 protein. Combining the data from in-house tissue microarrays and high-throughput datasets, we identified the expression level of CCDC80 in OVCA. We utilized cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm and single-sample gene set enrichment analysis (ssGSEA) to explore the relationship between CCDC80 and the tumor microenvironment (TME) landscape in OVCA. Pathway enrichment, function annotation, and transcription factor (TFs) exploration were conducted to study the latent molecular mechanisms. Moreover, the cell line data in the Genomics of Drug Sensitivity in Cancer (GDSC) database was used to discover the relationship between CCDC80 and drug sensitivity.Results: An integrated standard mean difference (SMD) of -0.919 (95% CI: -1.515-0.324, P = 0.002) identified the downregulation of CCDC80 in OVCA based on 1048 samples, and the sROC (AUC = 0.76) showed a moderate discriminatory ability of CCDC80 in OVCA. The fraction of infiltrating naive B cells showed significant differences between the high- and low-CCDC80 expression groups. Also, CCDC80-related genes are enriched in the Ras signaling pathway and metabolic of lipid. Nuclear receptor subfamily three group C member 1 (NR3C1) may be an upstream TF of CCDC80, and CCDC80 may be related to the sensitivity of mitocycin C and nilotinib.Conclusion: CCDC80 was downregulated in OVCA and may play a role as a tumor suppressor in OVCA.","PeriodicalId":13988,"journal":{"name":"International Journal of Genomics","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8608537/pdf/","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Genomics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2021/3752871","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 6

Abstract

Introduction: We aimed to explore the downregulation of the coiled-coil domain containing 80 (CCDC80) and its underlying molecular mechanisms in ovarian carcinoma (OVCA). Materials/Methods. Immunohistochemical staining was performed to confirm the expression status of CCDC80 protein. Combining the data from in-house tissue microarrays and high-throughput datasets, we identified the expression level of CCDC80 in OVCA. We utilized cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm and single-sample gene set enrichment analysis (ssGSEA) to explore the relationship between CCDC80 and the tumor microenvironment (TME) landscape in OVCA. Pathway enrichment, function annotation, and transcription factor (TFs) exploration were conducted to study the latent molecular mechanisms. Moreover, the cell line data in the Genomics of Drug Sensitivity in Cancer (GDSC) database was used to discover the relationship between CCDC80 and drug sensitivity.

Results: An integrated standard mean difference (SMD) of -0.919 (95% CI: -1.515-0.324, P = 0.002) identified the downregulation of CCDC80 in OVCA based on 1048 samples, and the sROC (AUC = 0.76) showed a moderate discriminatory ability of CCDC80 in OVCA. The fraction of infiltrating naive B cells showed significant differences between the high- and low-CCDC80 expression groups. Also, CCDC80-related genes are enriched in the Ras signaling pathway and metabolic of lipid. Nuclear receptor subfamily three group C member 1 (NR3C1) may be an upstream TF of CCDC80, and CCDC80 may be related to the sensitivity of mitocycin C and nilotinib.

Conclusion: CCDC80 was downregulated in OVCA and may play a role as a tumor suppressor in OVCA.

查看原文本刊更多论文

含80的coil - coil结构域在卵巢癌中的下调及其机制:一项综合研究。

引言：我们旨在探讨含有卷曲螺旋结构域80（CCDC80）在卵巢癌（OVCA）中的下调及其潜在的分子机制。材料/方法。免疫组化染色证实CCDC80蛋白的表达状态。结合来自内部组织微阵列和高通量数据集的数据，我们确定了CCDC80在OVCA中的表达水平。我们利用通过估计RNA转录物相对亚群的细胞类型鉴定（CIBERSORT）算法和单样本基因集富集分析（ssGSEA）来探索CCDC80与OVCA中肿瘤微环境（TME）景观之间的关系。通过通路富集、功能注释和转录因子（TF）探索来研究潜在的分子机制。此外，利用癌症药物敏感性基因组数据库（GDSC）中的细胞系数据来发现CCDC80与药物敏感性之间的关系。结果：基于1048个样本，-0.919的综合标准差（SMD）（95%可信区间：-1.515-0.324，P=0.002）确定了CCDC80在OVCA中的下调，sROC（AUC=0.76）显示CCDC80对OVCA的中等辨别能力。CCDC80高表达组和低表达组之间的浸润性幼稚B细胞的分数显示出显著差异。此外，CCDC80相关基因在Ras信号通路和脂质代谢中富集。核受体亚家族3族C成员1（NR3C1）可能是CCDC80的上游TF，CCDC80可能与有丝分裂素C和尼洛替尼的敏感性有关。结论：CCDC80在OVCA中表达下调，可能在OVCA的发生中起抑癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Genomics BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： International Journal of Genomics is a peer-reviewed, Open Access journal that publishes research articles as well as review articles in all areas of genome-scale analysis. Topics covered by the journal include, but are not limited to: bioinformatics, clinical genomics, disease genomics, epigenomics, evolutionary genomics, functional genomics, genome engineering, and synthetic genomics.