Nicotine associates to intracellular Mycobacterium tuberculosis inducing genes related with resistance to antimicrobial peptides.

IF 1.5 4区医学 Q3 RESPIRATORY SYSTEM

Experimental Lung Research Pub Date : 2021-12-01 Epub Date: 2021-11-22 DOI:10.1080/01902148.2021.2006829

Jeny de Haro-Acosta, Yolanda M Jacobo-Delgado, Adrian Rodríguez-Carlos, Flor Torres-Juárez, Zaida Araujo, Carmen J Serrano, Irma Gonzalez-Curiel, Rogelio Hernández-Pando, Eva Salinas, Bruno Rivas-Santiago

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引用次数: 1

Abstract

Tobacco consumption is related to an increased risk to develop tuberculosis. Antimicrobial peptides are essential molecules in the response to Mycobacterium tuberculosis (Mtb) because of their direct antimicrobial activity. The aim of this study was to demonstrate that nicotine enters into Mtb infected epithelial cells and associates with the mycobacteria inducing genes related to antimicrobial peptides resistance. Epithelial cells were infected with virulent Mtb, afterwards cells were stimulated with nicotine. The internalization of nicotine was followed using electron and confocal microscopy. The lysX expression was evaluated isolating mycobacterial RNA and submitted to RT-PCR analysis. Our results indicated that nicotine promotes Mtb growth in a dose-dependent manner in infected cells. We also reported that nicotine induces lysX expression. In conclusion, nicotine associates to intracellular mycobacteria promoting intracellular survival.

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尼古丁与细胞内结核分枝杆菌诱导基因相关的抗微生物肽抗性相关。

烟草消费与患肺结核的风险增加有关。由于抗菌肽具有直接的抗菌活性，因此在对结核分枝杆菌(Mtb)的反应中是必不可少的分子。本研究的目的是证明尼古丁进入结核分枝杆菌感染的上皮细胞，并与分枝杆菌诱导抗微生物肽耐药性相关的基因相关。上皮细胞感染强毒性结核分枝杆菌，然后用尼古丁刺激细胞。用电子显微镜和共聚焦显微镜观察烟碱内化情况。分离分枝杆菌RNA后检测lysX的表达并进行RT-PCR分析。我们的结果表明，尼古丁在感染细胞中以剂量依赖的方式促进结核分枝杆菌的生长。我们也报道了尼古丁诱导lysX的表达。总之，尼古丁与细胞内分枝杆菌有关，促进细胞内存活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental Lung Research 医学-呼吸系统

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Experimental Lung Research publishes original articles in all fields of respiratory tract anatomy, biology, developmental biology, toxicology, and pathology. Emphasis is placed on investigations concerned with molecular, biochemical, and cellular mechanisms of normal function, pathogenesis, and responses to injury. The journal publishes reports on important methodological advances on new experimental modes. Also published are invited reviews on important and timely research advances, as well as proceedings of specialized symposia. Authors can choose to publish gold open access in this journal.