Impact of tick-borne Anaplasma phagocytophilum infections in calves of moose (Alces alces) in southern Norway.

IF 1.5 4区 医学 Q3 PARASITOLOGY
Irma Razanske, Olav Rosef, Jana Radzijevskaja, Ricardas Krikstolaitis, Algimantas Paulauskas
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引用次数: 3

Abstract

The Gram-negative, obligate intracellular tick-transmitted pathogen Anaplasma phagocytophilum can cause acute febrile diseases in humans and domestic animals. The expansion of the tick Ixodes ricinus (Linnaeus, 1758) in northern Europe due to climate change is of serious concern for animal and human health. The aim of the present study was to investigate the impact of A. phagocytophilum infection in moose Alces alces (Linnaeus) calves by evaluating the carcass weights of infected and non-infected animals and examining animal tissues samples for co-infections with either species of Babesia Starcovici, 1893 or bacteria of the genus Bartonella. The carcasses of 68 free-ranging moose calves were weighed by hunters during the hunting seasons from 2014 to 2017 in two regions in southern Norway and spleen samples were collected. Anaplasma phagocytophilum was detected in moose sampled from locations infected with ticks with a prevalence of 82% (n = 46). The carcass weights of A. phagocytophilum-infected calves (n = 46) and non-infected (n = 22) calves were compared. Although the average weight of infected calves (45.6 kg) was lower than that of non-infected calves (46.5 kg), the difference was not statistically significant. Three different variants of the bacterium 16S rRNA gene were identified. The average weight of animals infected with variant I was 49.9 kg, whereas that of animals infected with variant III was 42.0 kg, but the difference was not statistically significant (p = 0.077). Co-infections of A. phagocytophilum with Bartonella spp. or with Babesia spp. were found in 20 and two calves, respectively. A triple infection was found in two calves. Sequence analysis of the 18S rRNA gene of Babesia-positive samples revealed the presence of Babesia cf. odocoilei (Emerson et Wright, 1970). Strains of Bartonella closely related to Bartonella bovis (Bermond, Boulouis, Heller, Laere, Monteil, Chomel, Sander, Dehio et Piemont, 2002) were identified based on phylogenetic analysis of the gltA and rpoB genes. The loss of body mass in moose calves in the tick-infected site was probably influenced by multiple factors.

挪威南部驼鹿幼崽(Alces Alces)中蜱传嗜吞噬细胞无原体感染的影响。
革兰氏阴性、专性细胞内蜱传病原体嗜吞噬细胞无原体可在人和家畜中引起急性发热性疾病。由于气候变化,北欧的蓖麻蜱(Linnaeus, 1758)的扩张严重影响了动物和人类的健康。本研究的目的是通过评估感染和未感染动物的胴体重量,并检查动物组织样本是否同时感染巴贝虫,1893或巴尔通体属细菌,来调查嗜吞噬细胞芽胞杆菌感染对驼鹿Alces Alces (Linnaeus)犊牛的影响。2014年至2017年,在挪威南部两个地区的狩猎季节,猎人对68只自由放养的驼鹿幼崽的尸体进行了称重,并收集了脾脏样本。在从蜱虫感染地点取样的驼鹿中检测到嗜吞噬细胞无原体,患病率为82% (n = 46)。比较吞噬细胞芽胞杆菌感染犊牛(n = 46)和未感染犊牛(n = 22)的胴体重。虽然感染小牛的平均体重(45.6公斤)低于未感染小牛的平均体重(46.5公斤),但差异没有统计学意义。鉴定出了细菌16S rRNA基因的三种不同变体。变异1的平均体重为49.9 kg,变异3的平均体重为42.0 kg,但差异无统计学意义(p = 0.077)。分别在20头犊牛和2头犊牛中发现嗜吞噬单胞杆菌与巴尔通体和巴贝斯虫共感染。在两头小牛身上发现了三重感染。巴贝斯虫阳性样本的18S rRNA基因序列分析显示存在巴贝斯虫(c.o docoilei) (Emerson et Wright, 1970)。通过gltA和rpoB基因的系统发育分析,鉴定出与牛巴尔通体亲缘关系较近的巴尔通体菌株(Bermond, boullouis, Heller, Laere, Monteil, Chomel, Sander, Dehio et Piemont, 2002)。在蜱虫感染区,驼鹿幼鹿的体重损失可能受到多种因素的影响。
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来源期刊
Folia Parasitologica
Folia Parasitologica 医学-寄生虫学
CiteScore
2.70
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.
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