The changing molecular epidemiology of Enterococcus faecium harbouring the van operon at a teaching hospital in Western Australia: A fifteen-year retrospective study

IF 4.5 3区医学 Q1 MICROBIOLOGY

International Journal of Medical Microbiology Pub Date : 2022-01-01 DOI:10.1016/j.ijmm.2021.151546

Terence Lee , Stanley Pang , Denise A. Daley , Julie C. Pearson , Sam Abraham , Geoffrey W. Coombs

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引用次数: 0

Abstract

Introduction

Enterococcus faecium is an opportunistic pathogen that has become one of the leading causes of hospital acquired infection that are resistant to multiple critically important antimicrobials.

Aim

The objective of the study was to describe the molecular characteristics and relationship between major strains of E. faecium harbouring the van operon and to determine if the strains had increasing virulence and antimicrobial resistance determinants over time.

Methods

E. faecium harbouring the van operon detected using PCR from surveillance rectal swabs of patients that were admitted to high-risk units at a Perth teaching hospital from 2001 to 2015 were retrospectively analysed using a whole genome sequencing and bioinformatics approach.

Results

ST18, ST78, ST80, ST173, ST203 and ST555 were identified as the major STs accounting for 93.7% of E. faecium isolates. Except for ST173, major STs identified at Royal Perth Hospital (RPH) have been reported across Australia and internationally. Isolates from each ST formed independently branched phylogenetic clusters with each harbouring unique virulence and antimicrobial resistance profiles. Depending on the ST, different genes conferring resistance to similar antimicrobial classes were identified. Except for ST80 which harboured the vanA type operon, all major strains harboured the vanB operon conferring only vancomycin resistance.

Conclusion

Major strains of E. faecium isolated over 15-years showed unique virulome and resistome profiles with no indication of increasing virulence or antimicrobial resistance determinants. Strains were distantly related and the acquisition of different genes encoding similar antimicrobial resistances suggest the independent evolution of each strain.

Data Summary

The whole genome sequences of all isolates from this study are accessible from the NCBI-SRA database under project number PRJNA575940 and PRJNA524213. Published reference sequence Aus0004 was obtained from NCBI-SRA under project number PRJNA86649 DOI:10.1128/JB.00259–12

查看原文本刊更多论文

西澳大利亚一所教学医院携带厢式腹腔脓包的粪肠球菌分子流行病学变化:一项15年回顾性研究

屎肠球菌是一种机会性病原体，已成为医院获得性感染的主要原因之一，对多种至关重要的抗菌素具有耐药性。目的研究携带van operon的主要粪肠杆菌的分子特征和相互关系，并确定菌株是否随时间增加毒力和耐药性决定因素。采用全基因组测序和生物信息学方法，对2001年至2015年在珀斯一家教学医院高风险病房住院的患者的直肠监测拭子中检测到的携带van operon的粪便进行回顾性分析。结果st18、ST78、ST80、ST173、ST203和ST555为主要STs，占粪肠杆菌分离株的93.7%。除ST173外，在皇家珀斯医院(RPH)发现的主要STs在澳大利亚和国际上都有报道。来自每个ST的分离物形成独立的分支系统发育簇，每个簇都具有独特的毒力和抗微生物抗性特征。根据ST，鉴定出对类似抗菌素类具有抗性的不同基因。除ST80携带vanB型操纵子外，其余主要菌株均携带vanB型操纵子，仅对万古霉素耐药。结论15年来分离的主要粪肠杆菌菌株表现出独特的病毒组和耐药组特征，没有显示出毒力或耐药决定因素增加的迹象。菌株亲缘关系较远，且获得编码相似耐药性的不同基因，表明每个菌株是独立进化的。本研究所有分离株的全基因组序列均可从NCBI-SRA数据库获取，项目编号为PRJNA575940和PRJNA524213。已发表的参考序列Aus0004来自NCBI-SRA，项目编号为PRJNA86649 DOI:10.1128/ JB.00259-12

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.