Effects of L-Theanine on Hepatic Ischemia-Reperfusion Injury in Rats.

IF 0.7 4区医学 Q4 TRANSPLANTATION

Experimental and Clinical Transplantation Pub Date : 2025-03-01 Epub Date: 2021-10-19 DOI:10.6002/ect.2021.0290

Hakan Küçükaslan, Arif Usta, Mehmet Uluşahin, Mustafa Emre Ercin, Huriye Patan, Birgül Vanizor Kural, Etem Alhan

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引用次数: 0

Abstract

Objectives: The effects of L-theanine on hepatic microcirculation during hepatic ischemia-reperfusion injury have not yet been investigated. The aim of this study was to investigate the influence of L-theanine on hepatic ischemia-reperfusion injury in rats.

Materials and methods: Thirty-two male Sprague Dawley rats weighing 250 to 300 g were used. Rats were divided into 4 groups: sham + saline, sham + L-theanine, hepatic ischemia-reperfusion injury + saline, and hepatic ischemia-reperfusion injury + L-theanine. Hepatic ischemia-reperfusion injury in rats was induced by 60 minutes of 70% ischemia and 4 hours of reperfusion. The extent of hepatic cell injury, functional capillary density, hepatic functions, and changes in some enzyme markers in hepatic tissue were investigated in the 4 groups.

Results: The induction of hepatic ischemia-reperfusion injury resulted in significant increases in hepatic necrosis; serum activity of alanine aminotransferase, lactate dehydrogenase, gamma-glutamyltransferase, and tumor necrosis factor alpha; tissue activity of inducible nitric oxide synthase, myeloperoxidase, and malondialdehyde, and oxide glutathione; and H score for hypoxia-inducible factor 1-alpha in the liver. In the liver, there were significant reductions in reduced glutathione, ratio of reduced glutathione-to-oxide glutathione, and functional capillary density. The use of L-theanine improved these changes.

Conclusions: L-theanine demonstrated protective effects on hepatic injury after ischemia-reperfusion injury in rats. However, new studies are needed to confirm the preventive or reducing effects of L-theanine on hepatic ischemia-reperfusion injury.

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L-茶氨酸对大鼠肝缺血再灌注损伤的影响

研究目的L-茶氨酸对肝缺血再灌注损伤过程中肝微循环的影响尚未得到研究。本研究旨在探讨左旋茶氨酸对大鼠肝缺血再灌注损伤的影响：使用 32 只体重为 250 至 300 克的雄性 Sprague Dawley 大鼠。大鼠分为 4 组：假肝 + 生理盐水组、假肝 + L-茶氨酸组、肝缺血再灌注损伤 + 生理盐水组和肝缺血再灌注损伤 + L-茶氨酸组。大鼠的肝缺血再灌注损伤是通过 60 分钟 70% 的缺血和 4 小时的再灌注诱发的。结果显示，4组大鼠的肝细胞损伤程度、功能性毛细血管密度、肝功能以及肝组织中一些酶标记物的变化均有不同程度的改变：结果：诱导肝缺血再灌注损伤导致肝坏死、血清中丙氨酸氨基转移酶、乳酸脱氢酶、γ-谷氨酰转移酶和肿瘤坏死因子α的活性、组织中诱导型一氧化氮合酶、髓过氧化物酶、丙二醛和氧化谷胱甘肽的活性以及肝脏中缺氧诱导因子1-α的H评分显著增加。在肝脏中，还原型谷胱甘肽、还原型谷胱甘肽与氧化型谷胱甘肽的比率以及功能性毛细血管密度均显著降低。使用左旋茶氨酸可改善这些变化：结论：左旋茶氨酸对大鼠缺血再灌注损伤后的肝损伤具有保护作用。结论：左旋茶氨酸对大鼠缺血再灌注损伤后的肝损伤具有保护作用，但还需要新的研究来证实左旋茶氨酸对肝缺血再灌注损伤的预防或减轻作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental and Clinical Transplantation 医学-移植

CiteScore

1.40

自引率

11.10%

发文量

258

审稿时长

6-12 weeks

期刊介绍： The scope of the journal includes the following: Surgical techniques, innovations, and novelties; Immunobiology and immunosuppression; Clinical results; Complications; Infection; Malignancies; Organ donation; Organ and tissue procurement and preservation; Sociological and ethical issues; Xenotransplantation.