Characterization of tick salivary gland and saliva alphagalactome reveals candidate alpha-gal syndrome disease biomarkers.

IF 3.8 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Expert Review of Proteomics Pub Date : 2021-12-01 Epub Date: 2022-01-11 DOI:10.1080/14789450.2021.2018305

Margarita Villar, Iván Pacheco, Lourdes Mateos-Hernández, Alejandro Cabezas-Cruz, Ala E Tabor, Manuel Rodríguez-Valle, Albert Mulenga, Katherine M Kocan, Edmour F Blouin, José de La Fuente

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引用次数: 11

Abstract

Background: Ticks are obligate hematophagous arthropods that synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) associated with the alpha-gal syndrome (AGS) or allergy to mammalian meat consumption.

Research design and methods: In this study, we used a proteomics approach to characterize tick proteins in salivary glands (sialome SG), secreted saliva (sialome SA) and with α-Gal modification (alphagalactome SG and SA) in model tick species associated with the AGS in the United States (Amblyomma americanum) and Australia (Ixodes holocyclus). Selected proteins reactive to sera (IgE) from patients with AGS were identified to advance in the identification of possible proteins associated with the AGS. For comparative analysis, the α-Gal content was measured in various tick species.

Results: The results confirmed that ticks produce proteins with α-Gal modifications and secreted into saliva during feeding. Proteins identified in tick alphagalactome SA by sera from patients with severe AGS symptomatology may constitute candidate disease biomarkers.

Conclusions: The results support the presence of tick-derived proteins with α-Gal modifications in the saliva with potential implications in AGS and other disorders and protective capacity against tick infestations and pathogen infection. Future research should focus on the characterization of the function of tick glycoproteins with α-Gal in tick biology and AGS.

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蜱唾液腺和唾液α -半乳糖的特征揭示了候选α -半乳糖综合征疾病生物标志物。

背景:蜱是专性食血节肢动物，其合成的多糖Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal)与α-Gal综合征(AGS)或对哺乳动物肉类过敏有关。研究设计与方法:本研究采用蛋白质组学方法对美国(Amblyomma americanum)和澳大利亚(Ixodes holocyclus)与AGS相关的模型蜱的唾液腺(sialome SG)、分泌唾液(sialome SA)和α-Gal修饰(alphagalactome SG和SA)中的蜱蛋白进行了研究。筛选AGS患者血清(IgE)反应蛋白，进一步确定可能与AGS相关的蛋白。为了进行比较分析，测定了不同蜱种α-Gal的含量。结果:证实蜱在取食过程中产生α-Gal修饰蛋白并分泌到唾液中。严重AGS患者血清中鉴定的蜱α半乳糖SA蛋白可能构成候选疾病生物标志物。结论:研究结果支持了唾液中存在α-Gal修饰的蜱源蛋白，该蛋白可能与AGS和其他疾病有关，并具有抵抗蜱虫侵染和病原体感染的保护能力。今后的研究重点应放在α-Gal糖蛋白在蜱生物学和AGS中的功能表征上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Proteomics 生物-生化研究方法

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.