Soluble total antigen derived from Toxoplasma gondii RH strain prevents apoptosis, but induces anti-apoptosis in human monocyte cell line.

IF 1.5 4区医学 Q3 PARASITOLOGY

Folia Parasitologica Pub Date : 2021-11-23 DOI:10.14411/fp.2021.026

Hanieh Mohammad Rahimi, Sara Nemati, Helia Alavifard, Kaveh Baghaei, Hamed Mirjalali, Mohammad Reza Zali

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引用次数: 1

Abstract

Apoptosis plays crucial role in the pathogenesis of toxoplasmosis, as it limits further development of the disease. The current study aimed to investigate the effects of different concentrations of soluble total antigen (STAg) of Toxoplasma gondii (Nicolle et Manceaux, 1908) on the apoptotic and anti-apoptotic pathways. PMA-activated THP-1 cell line was sensed by T. gondii STAg and the expression patterns of caspase-3, -7, -8, -9, Bax, Bcl-2, and Mcl-1 genes were evaluated. The results showed statistically significant concentration-dependent overexpression of both Bcl-2 (P-value < 0.0001) and Mcl-1 (P-value = 0.0147). The cas-7 showed overexpression in all concentrations (P-value < 0.0001). The cas-3 was suppressed in concentrations 100, 80, and 40 µg, but statistically significant downregulated in concentrations 10 and 20 µg. The Bax was suppressed in concentrations 100 to 20 µg, while it slightly downregulated 1.42 fold (P-value = 0.0029) in concentration 10 µg. The expression of cas-8 and -9 was suppressed in all concentrations. Our results indicated that T. gondii STAg downregulated and suppressed apoptotic and upregulated anti-apoptotic pathways. The upregulation of cas-7 in this study may indicate the role of T. gondii STAg in activation of inflammatory responses.

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刚地弓形虫RH株可溶性总抗原可抑制细胞凋亡，但可诱导单核细胞抗凋亡。

细胞凋亡在弓形虫病的发病机制中起着至关重要的作用，因为它限制了疾病的进一步发展。本研究旨在探讨不同浓度的刚地弓形虫可溶性总抗原(STAg) (Nicolle et Manceaux, 1908)对细胞凋亡和抗凋亡通路的影响。用弓形虫STAg检测pma激活的THP-1细胞株，检测caspase-3、-7、-8、-9、Bax、Bcl-2和Mcl-1基因的表达谱。结果显示Bcl-2和Mcl-1均呈浓度依赖性过表达(p值< 0.0001)，具有统计学意义(p值= 0.0147)。cas7在所有浓度下均呈过表达(p值< 0.0001)。cas3在浓度为100、80和40µg时被抑制，但在浓度为10和20µg时被下调，具有统计学意义。Bax在100 ~ 20µg浓度下被抑制，而在10µg浓度下则轻微下调1.42倍(p值= 0.0029)。在所有浓度下，cas8和-9的表达均被抑制。结果表明，弓形虫STAg下调和抑制细胞凋亡通路，上调抗凋亡通路。本研究中cas7的上调可能提示弓形虫STAg在炎症反应激活中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Parasitologica 医学-寄生虫学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.