Cutibacterium acnes phylogenetic type IC and II isolated from patients with non-acne diseases exhibit high-level biofilm formation

IF 4.5 3区医学 Q1 MICROBIOLOGY

International Journal of Medical Microbiology Pub Date : 2021-10-01 DOI:10.1016/j.ijmm.2021.151538

Keisuke Nakase , Juri Koizumi , Ren Midorikawa , Kento Yamasaki , Miho Tsutsui , Sae Aoki , Yutaka Nasu , Yuji Hirai , Hidemasa Nakaminami , Norihisa Noguchi

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引用次数: 11

Abstract

Cutibacterium (formerly Propionibacterium) acnes is an important for not only exacerbating factor of acne vulgaris but also pathogen of surgical site infections (SSIs) in orthopedics and plastic surgery. Although biofilm-forming (BF) C. acnes are associated with intractable SSI, characteristics of these strains were still unknown. Here, we explored detailed molecular epidemiological features of BF C. acnes isolated as causative pathogen of infectious diseases. Phylogenetic types of 205 C. acnes strains isolated between 2013 and 2018 from 18 clinical departments of a university hospital in Japan were determined by single-locus sequence type (SLST). Clade H (traditional type IC) and K (type II) which are less relevant with healthy skin and acne vulgaris, were detected in 26.8% (55/205) and 16.1% (33/205) of the strains, respectively. The incidence of them was significantly higher than that of acne patients (H and K, each 2.9%, P < 0.05). In addition, SLST distribution of C. acnes strains differed by each department and isolation site. When biofilm formation was quantified, 51 strains (24.9%) were defined as high-BF strains. Notably, most high-BF strains were classified into the strains of clade H (56.4%, 31/55) and clade K (54.4%, 18/33), and these strains were frequently found in the strains isolated from patients of medical emergency center and plastic surgery. Similarly, high-BF strains were frequently found among the isolates from blood (35.7%) and catheters (30.0%), with a high proportion belonging to clades H and K. Compared to C. acnes strains isolated from acne patients, antimicrobial-resistant strains were less identified in non-acne patients. Our findings showed that pathogenicity of C. acnes strains differs by their phylogenetic types. Furthermore, we showed clade H and K have the ability of high biofilm formation and suggest that these strains have potential to become a risk factor for SSI.

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从非痤疮疾病患者中分离出的痤疮表皮杆菌系统发育型IC和II表现出高水平的生物膜形成

痤疮表皮杆菌(原丙酸杆菌)不仅是寻常性痤疮的加重因素，也是整形外科手术部位感染的重要病原体。虽然生物膜形成(BF) C. acnes与顽固性SSI有关，但这些菌株的特征仍然未知。本文对感染性疾病病原菌——痤疮BF C. acnes的分子流行病学特征进行了详细的探讨。采用单位点序列分型(SLST)方法对2013 - 2018年从日本某大学医院18个临床科室分离的205株痤疮乳杆菌进行系统发育分型分析。与健康皮肤和寻常性痤疮相关性较低的分支H(传统IC型)和K (II型)分别占26.8%(55/205)和16.1%(33/205)。其发生率显著高于痤疮患者(H和K，各2.9%，P <0.05)。此外，不同科室、不同分离部位的痤疮C. acnes菌株的SLST分布也存在差异。定量测定生物膜形成时，51株(24.9%)为高bf菌株。值得注意的是，大多数高bf菌株属于H支(56.4%，31/55)和K支(54.4%，18/33)，这些菌株常见于医疗急救中心和整形外科患者分离的菌株。同样，从血液和导管中分离出的高bf菌株(35.7%)和高bf菌株(30.0%)也很常见，其中H和k支系的比例很高，与从痤疮患者分离出的痤疮C.菌株相比，在非痤疮患者中分离出的耐药菌株较少。结果表明，不同系统发育类型的菌株致病性不同。此外，我们发现进化枝H和K具有高生物膜形成能力，并表明这些菌株有可能成为SSI的危险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.