Modulation of neo-endothelialization of vascular graft materials by silk fibroin.

IF 1.3 4区医学 Q4 ENGINEERING, BIOMEDICAL

Biomedical Engineering / Biomedizinische Technik Pub Date : 2021-10-11 Print Date: 2021-12-20 DOI:10.1515/bmt-2020-0238

Congcong Zhan, Chuanjun Xia, Pengfei Wang, Pingdeng Ming, Shanfeng Zhang, Junying Chen, Xia Huang

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引用次数: 0

Abstract

Controlled neo-endothelialization is critical to the patency of vascular grafts. Expanded polyethylene terephthalate (PET) vascular grafts were grafted with polyethylene glycol (PEG), irradiated with ultraviolet light, and subsequently coated with silk fibroin (SF) and EDC in a dip-coating process. Endothelial cells were cultivated on the coated samples for 1, 3, 5, and 7 days, and characterized by fluorescence microscopy and scanning electron microscopy (SEM). The quantitative analyse of CCK-8 method was used to assess ECs proliferation. The results reveal the correlation between grafting components and cell adhesion. We demonstrated that PET with SF grafting facilitated cell adhesion and spreading. Following 7 days of cell culture in vitro, PET-PEG6000-SF (PEG molecular weight 6,000) displayed spreading of cells over a significantly larger area. Rapid endothelialization on a modified PET surface resulted in large tissue pack that can be observed by SEM.

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丝素蛋白对血管移植材料新内皮化的调节作用。

可控的新内皮化是血管移植通畅的关键。用聚乙二醇(PEG)接枝膨胀聚对苯二甲酸乙二醇酯(PET)血管移植物，用紫外光照射，然后用丝素(SF)和EDC浸渍涂覆。内皮细胞在包被的样品上培养1、3、5和7天，并通过荧光显微镜和扫描电镜(SEM)对其进行表征。采用CCK-8法定量分析细胞增殖情况。结果揭示了接枝组分与细胞粘附的相关性。我们证明了PET与SF嫁接促进了细胞的粘附和扩散。细胞体外培养7天后，PET-PEG6000-SF (PEG分子量为6000)显示细胞在更大的区域内扩散。在修饰的PET表面上快速内皮化导致可以通过扫描电镜观察到的大组织包。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Engineering / Biomedizinische Technik 医学-工程：生物医学

CiteScore

3.50

自引率

5.90%

发文量

审稿时长

2-3 weeks

期刊介绍： Biomedical Engineering / Biomedizinische Technik (BMT) is a high-quality forum for the exchange of knowledge in the fields of biomedical engineering, medical information technology and biotechnology/bioengineering. As an established journal with a tradition of more than 60 years, BMT addresses engineers, natural scientists, and clinicians working in research, industry, or clinical practice.