Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2022-01-01 DOI:10.1007/7651_2021_427

Arline Joachim, Shiqiao Ye, Ming-Tao Zhao

引用次数: 2

Abstract

The generation of cardiomyocytes (CMs) and endothelial cells (ECs) from human induced pluripotent stem cells (iPSCs) allows for precise modeling of cardiovascular disease using clinically relevant and patient-specific cells. Differentiation of human iPSCs into cardiomyocytes (iPSC-CMs) and endothelial cells (iPSC-ECs) is governed by small molecules that regulate the WNT signaling pathway. Here we outline the detailed steps to generate iPSC-CMs and iPSC-ECs through small molecule-mediated monolayer differentiation.

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通过Wnt信号的化学调节，人类iPSCs生成心肌细胞和内皮细胞。

从人诱导多能干细胞(iPSCs)生成心肌细胞(CMs)和内皮细胞(ECs)允许使用临床相关和患者特异性细胞精确建模心血管疾病。人类iPSCs分化为心肌细胞(iPSC-CMs)和内皮细胞(iPSC-ECs)是由调节WNT信号通路的小分子调控的。在这里，我们概述了通过小分子介导的单层分化生成iPSC-CMs和iPSC-ECs的详细步骤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.