Analytical validation of a point-of-care test and an automated immunoturbidimetric assay for the measurement of canine C-reactive protein in serum.

IF 1.1 4区 农林科学 Q2 Veterinary
Marshal A Covin, Robynne R Gomez, Jan S Suchodolski, Jörg M Steiner, Jonathan A Lidbury
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Abstract

C-reactive protein (CRP) is an acute phase protein, which is used to evaluate and monitor the response of the innate immune system to a variety of inflammatory processes in the dog. The purpose of this study was to analytically validate a point-of-care assay (IDEXX Catalyst CRP Test) and an immunoturbidimetric assay (Gentian Canine CRP Immunoassay) for the measurement of serum CRP concentrations in dogs. These 2 assays (Catalyst, Gentian) were compared to a previously validated enzyme-linked immunosorbent assay (Tridelta Development EIA Canine CRP Assay). Linearity, precision, reproducibility, and accuracy were assessed using leftover serum samples. Agreement between assays was assessed using leftover serum samples and serum from clinically healthy dogs. Observed to expected ratios (O/E) for dilutional parallelism were 83.9 to 163.1% and 108.3 to 160.6% for the Catalyst and the Gentian assays, respectively. Coefficients of variation for intra-assay variability ranged from 6.4 to 9.5% for the Catalyst assay and 1.5 to 2.6% for the Gentian assay. Coefficients of variation for inter-assay variability ranged from 3.8 to 18.2% for the Catalyst assay and 4.5 to 5.8% for the Gentian assay. The mean O/E for recovery were 97.9% and 98.5% for the Catalyst and Gentian assays, respectively. Correlations between assays were as follows: Catalyst and Tridelta (R 2 = 0.76), Gentian and Tridelta (R 2 = 0.79), and Catalyst and Gentian (R 2 = 0.98). The Catalyst and Gentian assays are both acceptable for measuring CRP in dog serum, but their results are not directly comparable with the Tridelta assay.

Abstract Image

分析验证点护理测试和自动免疫比浊法测定血清中犬c反应蛋白。
c反应蛋白(CRP)是一种急性期蛋白,用于评估和监测犬的先天免疫系统对各种炎症过程的反应。本研究的目的是分析验证一种即时检测法(IDEXX Catalyst CRP Test)和一种免疫比浊法(龙胆草犬CRP免疫测定法)对狗血清CRP浓度的测量。将这两种检测方法(Catalyst,龙胆草)与之前验证的酶联免疫吸附检测方法(Tridelta Development EIA犬CRP检测)进行比较。使用剩余血清样本评估线性度、精密度、重现性和准确性。使用剩余的血清样本和临床健康犬的血清来评估测定之间的一致性。观察到稀释平行度的预期比值(O/E)在Catalyst和龙胆草试验中分别为83.9 ~ 163.1%和108.3 ~ 160.6%。催化剂测定法的测定内变异系数为6.4 - 9.5%,龙胆测定法为1.5 - 2.6%。催化剂测定法的测定间变异系数为3.8 - 18.2%,龙胆草测定法为4.5 - 5.8%。催化剂和龙胆草的平均回收率分别为97.9%和98.5%。各测定指标的相关性为:Catalyst与Tridelta (r2 = 0.76)、龙胆草与Tridelta (r2 = 0.79)、Catalyst与龙胆草(r2 = 0.98)。Catalyst和龙胆草试验都可用于狗血清中CRP的测定,但其结果不能与Tridelta试验直接比较。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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