Mohammed Quader, Renee Cholyway, Niluka Wickramaratne, Oluwatoyin Akande, Martin Mangino, Eleonora Mezzaroma, Adolfo G Mauro, Qun Chen, Alexander Kantlis, Stefano Toldo
{"title":"Refining murine heterotopic heart transplantation: A model to study ischemia and reperfusion injury in donation after circulatory death hearts.","authors":"Mohammed Quader, Renee Cholyway, Niluka Wickramaratne, Oluwatoyin Akande, Martin Mangino, Eleonora Mezzaroma, Adolfo G Mauro, Qun Chen, Alexander Kantlis, Stefano Toldo","doi":"10.1002/ame2.12176","DOIUrl":null,"url":null,"abstract":"<p><p>Heart transplantation is a lifesaving procedure, which is limited by the availability of donor hearts. Using hearts from donors after circulatory death, which have sustained global ischemia, requires thorough studies on reliable and reproducible models that developing researchers may not have mastered. By combining the most recent literature and our recommendations based on observations and trials and errors, the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied, and thus allowing the scientific community to advance organ preservation research. Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.</p>","PeriodicalId":7840,"journal":{"name":"Animal Models and Experimental Medicine","volume":"4 3","pages":"283-296"},"PeriodicalIF":0.0000,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ame2.12176","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Models and Experimental Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/ame2.12176","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Heart transplantation is a lifesaving procedure, which is limited by the availability of donor hearts. Using hearts from donors after circulatory death, which have sustained global ischemia, requires thorough studies on reliable and reproducible models that developing researchers may not have mastered. By combining the most recent literature and our recommendations based on observations and trials and errors, the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied, and thus allowing the scientific community to advance organ preservation research. Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.
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