Correlation between magnetic resonance, X-ray imaging alterations and histological changes in an ovine model of age-related disc degeneration.

IF 3.2 3区医学 Q3 CELL & TISSUE ENGINEERING

European cells & materials Pub Date : 2021-09-24 DOI:10.22203/eCM.v042a13

N Bouhsina, C Decante, J B Hardel, S Madec, J Abadie, A Hamel, C Le Visage, J Lesoeur, J Guicheux, J Clouet, M Fusellier

{"title":"Correlation between magnetic resonance, X-ray imaging alterations and histological changes in an ovine model of age-related disc degeneration.","authors":"N Bouhsina, C Decante, J B Hardel, S Madec, J Abadie, A Hamel, C Le Visage, J Lesoeur, J Guicheux, J Clouet, M Fusellier","doi":"10.22203/eCM.v042a13","DOIUrl":null,"url":null,"abstract":"<p><p>Sheep are one of the many animal models used to investigate the pathophysiology of disc degeneration and the regenerative strategies for intervertebral disc (IVD) disease. To date, few studies have thoroughly explored ageing of ovine lumbar IVDs. Hence, the objective of the present study was to concomitantly assess the development of spontaneous age-related lumbar IVD degeneration in sheep using X-ray, magnetic resonance imaging (MRI) as well as histological analyses. 8 young ewes (< 48 months old) and 4 skeletally mature ewes (> 48 months old) were included. Disc height, Pfirrmann and modified Pfirrmann grades as well as T2-wsi and T2 times were assessed by X-ray and MRI. The modified Boos score was also determined using histology sections. Pfirrmann (2 to 3) and modified Pfirrmann (2 to 4) grades as well as Boos scores (7 to 13) gradually increased with ageing, while T2-weighted signal intensity (1.18 to 0.75), T2 relaxation time (114.36 to 70.65 ms) and disc height (4.1 to 3.2 mm) decreased significantly. All the imaging modalities strongly correlated with the histology (p < 0.0001). The present study described the suitability of sheep as a model of age-related IVD degeneration by correlation of histological tissue alterations with the changes observed using X-ray and MRI. Given the structural similarities with humans, the study demonstrated that sheep warrant being considered as a pertinent animal model to investigate IVD regenerative strategies without induction of degeneration.</p>","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"166-178"},"PeriodicalIF":3.2000,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European cells & materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.22203/eCM.v042a13","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}

引用次数: 4

Abstract

Sheep are one of the many animal models used to investigate the pathophysiology of disc degeneration and the regenerative strategies for intervertebral disc (IVD) disease. To date, few studies have thoroughly explored ageing of ovine lumbar IVDs. Hence, the objective of the present study was to concomitantly assess the development of spontaneous age-related lumbar IVD degeneration in sheep using X-ray, magnetic resonance imaging (MRI) as well as histological analyses. 8 young ewes (< 48 months old) and 4 skeletally mature ewes (> 48 months old) were included. Disc height, Pfirrmann and modified Pfirrmann grades as well as T2-wsi and T2 times were assessed by X-ray and MRI. The modified Boos score was also determined using histology sections. Pfirrmann (2 to 3) and modified Pfirrmann (2 to 4) grades as well as Boos scores (7 to 13) gradually increased with ageing, while T2-weighted signal intensity (1.18 to 0.75), T2 relaxation time (114.36 to 70.65 ms) and disc height (4.1 to 3.2 mm) decreased significantly. All the imaging modalities strongly correlated with the histology (p < 0.0001). The present study described the suitability of sheep as a model of age-related IVD degeneration by correlation of histological tissue alterations with the changes observed using X-ray and MRI. Given the structural similarities with humans, the study demonstrated that sheep warrant being considered as a pertinent animal model to investigate IVD regenerative strategies without induction of degeneration.

查看原文本刊更多论文

羊年龄相关性椎间盘退变模型的磁共振、x线成像改变与组织学改变的相关性。

绵羊是用于研究椎间盘退变病理生理和椎间盘疾病再生策略的众多动物模型之一。迄今为止，很少有研究深入探讨了绵羊腰椎ivd的老化。因此，本研究的目的是利用x射线、磁共振成像(MRI)和组织学分析来同时评估绵羊自发性年龄相关性腰椎IVD变性的发展。试验包括8只幼母羊(< 48月龄)和4只骨骼成熟母羊(> 48月龄)。通过x线和MRI评估椎间盘高度、Pfirrmann和改良Pfirrmann分级以及T2-wsi和T2时间。修改后的Boos评分也通过组织学切片确定。随着年龄的增长，Pfirrmann(2 ~ 3)和改良Pfirrmann(2 ~ 4)评分以及Boos评分(7 ~ 13)逐渐升高，T2加权信号强度(1.18 ~ 0.75)、T2松弛时间(114.36 ~ 70.65 ms)和椎间盘高度(4.1 ~ 3.2 mm)显著降低。所有影像学表现均与组织学高度相关(p < 0.0001)。本研究描述了羊作为年龄相关性IVD变性模型的适用性，通过x射线和MRI观察到组织学组织改变的相关性。考虑到与人类的结构相似性，该研究表明绵羊可以作为研究IVD再生策略而不诱导变性的相关动物模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

European cells & materials 生物-材料科学：生物材料

CiteScore

6.00

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： eCM provides an interdisciplinary forum for publication of preclinical research in the musculoskeletal field (Trauma, Maxillofacial (including dental), Spine and Orthopaedics). The clinical relevance of the work must be briefly mentioned within the abstract, and in more detail in the paper. Poor abstracts which do not concisely cover the paper contents will not be sent for review. Incremental steps in research will not be entertained by eCM journal.Cross-disciplinary papers that go across our scope areas are welcomed.