Molecular Characterization, Virulence and Antimicrobial Susceptibility Testing of Riemerella anatipestifer Isolated from Ducklings.

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2021-01-01 DOI:10.4265/bio.26.181

Amany Shousha, Amal Awad, Gamal Younis

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引用次数: 8

Abstract

This pilot study aimed to characterize Riemerella anatipestifer from ducklings, testing their susceptibility to antimicrobial agents and to detect their virulence markers. Seven R. anatipestifer isolates with 11.67% infection rate were identified out of sixty freshly dead ducklings and confirmed by PCR assay targeting gyrB gene. The gyrB gene sequences of R. anatipestifer isolates were 100% identical to each other and also showed 100% sequence similarity to the published gyrB genes. Four virulence genes namely ompA, prtC, hagA, and sspA were identified in all isolates except sspA was detected in 5 isolates. The antibiogram revealed higher sensitive to imipenem, amikacin, and rifampin, while, a remarkably high resistance was displayed against ampicillin, penicillin, cefipime, trimethoprim/sulfamethoxazole, gentamicin, ceftazidime, streptomycin and cefoperazone. Proper and rapid identification of R. anatipestifer with detection of their antimicrobial susceptibility and its virulence potential is essential for understanding the epidemiology of R. anatipestifer and to apply the effective control strategies.

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鸭疫里默氏菌分离株分子特征、毒力及药敏试验

本初步研究旨在鉴定鸭疫里默氏菌的特征，测试其对抗菌药物的敏感性，并检测其毒力标记物。从60只新鲜死亡雏鸭中分离出7株鸭疫鼠，感染率为11.67%。禽疫鼠分离株gyrB基因序列完全相同，与已发表的gyrB基因序列相似度也达到100%。除5株菌株检测到sspA外，其余菌株均检测到ompA、prtC、hagA和sspA 4种毒力基因。抗生素谱显示对亚胺培南、阿米卡星、利福平敏感性较高，对氨苄西林、青霉素、头孢吡肟、甲氧苄啶/磺胺甲恶唑、庆大霉素、头孢他啶、链霉素、头孢哌酮耐药较高。正确、快速地鉴定鸭口疫鼠，检测其药敏和潜在毒力，对了解鸭口疫鼠的流行病学和采取有效的控制策略至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.