IgG4-specific responses in patients with Staphylococcus aureus bone infections are not predictive of postoperative complications.

IF 3.2 3区 医学 Q3 CELL & TISSUE ENGINEERING
J R Owen, M P Campbell, M D Mott, C A Beck, C Xie, G Muthukrishnan, J L Daiss, E M Schwarz, S L Kates
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引用次数: 1

Abstract

The most prevalent pathogen in bone infections is Staphylococcus aureus; its incidence and severity are partially determined by host factors. Prior studies showed that anti-glucosaminidase (Gmd) antibodies are protective in animals, and 93.3 % of patients with culture-confirmed S. aureus osteomyelitis do not have anti-Gmd levels > 10 ng/mL in serum. Infection in patients with high anti-Gmd remains unexplained. Are anti-Gmd antibodies in osteomyelitis patients of the non-opsonising, non-complement-fixing IgG4 isotype? The relative amounts of IgG4 and total IgG against Gmd and 7 other S. aureus antigens: iron-surface determinants (Isd) IsdA, IsdB, and IsdH, amidase (Amd), α-haemolysin (Hla), chemotaxis inhibitory protein from S. aureus (CHIPS), and staphylococcal-complement inhibitor (SCIN) were determined in sera from healthy controls (Ctrl, n = 92), osteomyelitis patients whose surgical treatment resulted in infection control (IC, n = 95) or an adverse outcome (AD, n = 40), and post-mortem (PM, n = 7) blood samples from S. aureus septic-death patients. Anti-Gmd IgG4 levels were generally lower in infected patients compared to controls; however, levels among the infected were higher in AD than IC patients. Anti-IsdA, IsdB and IsdH IgG4 levels were increased in infected patients versus controls, and Jonckheere-Terpstra tests of levels revealed an increasing order of infection (Ctrl < IC < AD < PM) for anti-Isd IgG4 antibodies and a decreasing order of infection (Ctrl > IC > AD > PM) for anti-autolysin (Atl) IgG4 antibodies. Collectively, this does not support an immunosuppressive role of IgG4 in S. aureus osteomyelitis but is consistent with a paradigm of high anti-Isd and low anti-Atl responses in these patients.

Abstract Image

Abstract Image

Abstract Image

金黄色葡萄球菌骨感染患者的igg4特异性反应不能预测术后并发症。
骨感染中最常见的病原体是金黄色葡萄球菌;其发病率和严重程度部分取决于宿主因素。先前的研究表明,抗葡萄糖苷酶(Gmd)抗体在动物中具有保护作用,93.3%的培养确诊金黄色葡萄球菌骨髓炎患者血清中抗Gmd水平不> 10 ng/mL。高抗gmd患者的感染仍未得到解释。骨髓炎患者的抗gmd抗体是非调理、非补体固定的IgG4同型吗?IgG4和总IgG对Gmd及其他7种金黄色葡萄球菌抗原的相对量:对健康对照(对照组,n = 92)、手术治疗后感染得到控制的骨髓炎患者(对照组,n = 95)或出现不良结果的骨髓炎患者(AD, n = 40)和金黄色葡萄球菌败血症死亡患者的死后血液样本(PM, n = 7)进行了铁表面决定因子(Isd)、IsdA、IsdB和IsdH、酰胺酶(Amd)、α-溶血素(Hla)、趋化抑制蛋白(CHIPS)和葡萄球菌补体抑制剂(SCIN)的检测。与对照组相比,感染患者的抗gmd IgG4水平普遍较低;然而,AD感染者的水平高于IC患者。与对照组相比,感染患者的抗isda、IsdB和IsdH IgG4水平升高,jonckheer - terpstra检测显示,抗isd IgG4抗体的感染顺序为递增(Ctrl < IC < AD < PM),抗自溶素(Atl) IgG4抗体的感染顺序为递减(Ctrl > IC > AD > PM)。总的来说,这并不支持IgG4在金黄色葡萄球菌骨髓炎中的免疫抑制作用,但与这些患者的高抗isd和低抗atl反应的范式一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
European cells & materials
European cells & materials 生物-材料科学:生物材料
CiteScore
6.00
自引率
6.50%
发文量
55
审稿时长
1.5 months
期刊介绍: eCM provides an interdisciplinary forum for publication of preclinical research in the musculoskeletal field (Trauma, Maxillofacial (including dental), Spine and Orthopaedics). The clinical relevance of the work must be briefly mentioned within the abstract, and in more detail in the paper. Poor abstracts which do not concisely cover the paper contents will not be sent for review. Incremental steps in research will not be entertained by eCM journal.Cross-disciplinary papers that go across our scope areas are welcomed.
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