IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-01-01 Epub Date: 2021-09-20 DOI:10.5603/FHC.a2021.0019

Wei Xiong, Hua Chen, Jiequan Lu, Jie Ren, Chen Nie, Ruijuan Liang, Feng Liu, Baofeng Huang, Yu Luo

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引用次数: 3

Abstract

Introduction: The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that the serum level of IL-39 significantly increased in patients with acute myocardial infarction. However, the role of IL-39 in cardiomyocyte apoptosis remains unclear.

Material and methods: In this study, the cultured mouse HL-1 cardiomyocytes were incubated with PBS, 0-100 ng/mL IL-39, 200 μM H2O2 or 20 μM Trolox.

Results: IL-39 promoted the production of intracellular reactive oxygen species (ROS) in a concentration dependent manner in HL-1 cardiomyocytes. IL-39 and H2O2 both significantly promoted the production of intracellular ROS, increased the level of intracellular CCL2, stimulated the apoptotic progress of cardiomyocytes, increased the mRNA and protein expression levels of Bax, caspase-3, and p-p38 MAPK, and decreased the mRNA and protein expression levels of Bcl-2. ROS production, CCL2 level, cardiomyocyte apoptosis, and expression of Bax, caspase-3, and p-p38 MAPK were significantly amplified by the administration of IL-39 combined with H2O2, and these processes were significantly alleviated by an antioxidant Trolox.

Conclusion: This study was novel in revealing that IL-39 promoted apoptosis by stimulating the phosphorylation of p38 MAPK in mouse HL-1 cardiomyocytes.

查看原文本刊更多论文

IL-39增加ROS的产生，促进凋亡心肌细胞p38 MAPK的磷酸化。

细胞因子白介素(IL)-39是IL-12家族的新成员。我们前期研究发现急性心肌梗死患者血清IL-39水平明显升高。然而，IL-39在心肌细胞凋亡中的作用尚不清楚。材料与方法:将培养的小鼠HL-1心肌细胞分别用PBS、0-100 ng/mL IL-39、200 μM H2O2或20 μM Trolox孵育。结果:IL-39促进HL-1心肌细胞细胞内活性氧(ROS)的产生，并呈浓度依赖性。IL-39和H2O2均显著促进细胞内ROS的产生，增加细胞内CCL2水平，刺激心肌细胞凋亡进程，提高Bax、caspase-3、p-p38 MAPK mRNA和蛋白表达水平，降低Bcl-2 mRNA和蛋白表达水平。IL-39与H2O2联合作用可显著增加ROS的产生、CCL2水平、心肌细胞凋亡以及Bax、caspase-3和p-p38 MAPK的表达，而抗氧化剂Trolox可显著缓解这些过程。结论:本研究新颖地揭示了IL-39通过刺激小鼠HL-1心肌细胞p38 MAPK的磷酸化而促进细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.