Atg8ylation as a general membrane stress and remodeling response.

IF 4.1 Q2 CELL BIOLOGY

Cell Stress Pub Date : 2021-08-12 eCollection Date: 2021-09-01 DOI:10.15698/cst2021.09.255

Suresh Kumar, Jingyue Jia, Vojo Deretic

引用次数: 0

Abstract

The yeast Atg8 protein and its paralogs in mammals, mammalian Atg8s (mAtg8s), have been primarily appreciated for their participation in autophagy. However, lipidated mAtg8s, including the most frequently used autophagosomal membrane marker LC3B, are found on cellular membranes other than autophagosomes. Here we put forward a hypothesis that the lipidation of mAtg8s, termed 'Atg8ylation', is a general membrane stress and remodeling response analogous to the role that ubiquitylation plays in tagging proteins. Ubiquitin and mAtg8s are related in sequence and structure, and the lipidation of mAtg8s occurs on its C-terminal glycine, akin to the C-terminal glycine of ubiquitin. Conceptually, we propose that mAtg8s and Atg8ylation are to membranes what ubiquitin and ubiquitylation are to proteins, and that, like ubiquitylation, Atg8ylation has a multitude of downstream effector outputs, one of which is autophagy.

Abstract Image

查看原文本刊更多论文

Atg8ylation 是一种普遍的膜应激和重塑反应。

酵母Atg8蛋白及其在哺乳动物中的类似物哺乳动物Atg8s（mAtg8s）主要因参与自噬而受到关注。然而，脂质化的 mAtg8s（包括最常用的自噬体膜标记 LC3B）存在于自噬体以外的细胞膜上。在这里，我们提出了一个假设，即被称为 "Atg8ylation "的mAtg8s脂质化是一种普遍的膜应激和重塑反应，类似于泛素化在标记蛋白质中所起的作用。泛素和 mAtg8s 在序列和结构上是相关的，mAtg8s 的脂化发生在其 C 端甘氨酸上，与泛素的 C 端甘氨酸相似。从概念上讲，我们认为 mAtg8s 和 Atg8ylation 对膜的作用就像泛素和泛素化对蛋白质的作用一样，而且与泛素化一样，Atg8ylation 也有多种下游效应输出，自噬就是其中之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell Stress Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

13.50

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： Cell Stress is an open-access, peer-reviewed journal that is dedicated to publishing highly relevant research in the field of cellular pathology. The journal focuses on advancing our understanding of the molecular, mechanistic, phenotypic, and other critical aspects that underpin cellular dysfunction and disease. It specifically aims to foster cell biology research that is applicable to a range of significant human diseases, including neurodegenerative disorders, myopathies, mitochondriopathies, infectious diseases, cancer, and pathological aging. The scope of Cell Stress is broad, welcoming submissions that represent a spectrum of research from fundamental to translational and clinical studies. The journal is a valuable resource for scientists, educators, and policymakers worldwide, as well as for any individual with an interest in cellular pathology. It serves as a platform for the dissemination of research findings that are instrumental in the investigation, classification, diagnosis, and therapeutic management of major diseases. By being open-access, Cell Stress ensures that its content is freely available to a global audience, thereby promoting international scientific collaboration and accelerating the exchange of knowledge within the research community.