Comparison of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption.

Q1 Biochemistry, Genetics and Molecular Biology
Stem cell investigation Pub Date : 2021-08-16 eCollection Date: 2021-01-01 DOI:10.21037/sci-2020-039
Meghna Bhandary, Shama Rao, Alandur Veena Shetty, Basavarajappa Mohana Kumar, Amitha Mahesh Hegde, Rachaita Chhabra
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引用次数: 1

Abstract

Background: Stem cells from human exfoliated deciduous teeth (SHED) are regarded as an attractive cell source for tissue regeneration. However, the effect of different levels of root resorption on the characteristics of SHED remains less understood. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined. To compare cellular and biological characteristics of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption.

Methods: The pulp was obtained from the deciduous posterior teeth depending on the level of root resorption, and isolated SHEDs were grouped as follows: Teeth with 0 to 1/3rd root resorption as SHEDs (G1) and 1/3rd to 2/3rd root resorption as SHEDs (G2). Teeth were also collected from >2/3rd root resorption status, but failed to establish primary culture of SHED as the availability of pulp tissue was too less. Later, isolated SHEDs were compared on their morphology, viability, growth kinetics, colony-forming ability, expression of cell surface markers and in vitro differentiation into osteocytes and adipocytes.

Results: No major differences were observed in terms of cellular morphology, viability, proliferation rate, colony-forming ability, cell surface markers expression, and mesenchymal lineage differentiation of SHEDs isolated from posterior teeth with 0 to 1/3rd and 1/3rd to 2/3rd root resorption. However, SHED from teeth with 0 to 1/3rd root resorption (G1) displayed relatively higher proliferation capacity and expression of selected markers.

Conclusions: Collectively, SHEDs (G1) and SHEDs (G2) showed comparable cellular and biological characteristics that enable their possible applications in regenerative therapies.

Abstract Image

不同根吸收水平的人脱落乳牙后牙干细胞的比较。
背景:人脱落乳牙(SHED)干细胞被认为是一种有吸引力的组织再生细胞来源。然而,不同水平的根系吸收对SHED特性的影响尚不清楚。因此,需要确定最适合隔离车棚的齿源。比较不同根吸收水平的人脱落后乳牙干细胞的细胞和生物学特性。方法:根据根吸收的程度,从乳牙后牙中取出牙髓,将离体舍分为:根吸收0 ~ 1/3的牙舍(G1)和根吸收1/3 ~ 2/3的牙舍(G2)。牙根吸收状态也大于2/3,但由于牙髓组织的可用性过低,未能建立SHED的原代培养。比较离体shed的形态、活力、生长动力学、集落形成能力、细胞表面标志物的表达以及向骨细胞和脂肪细胞的体外分化。结果:0 ~ 1/3根吸收和1/3 ~ 2/3根吸收后牙分离的舍在细胞形态、活力、增殖率、集落形成能力、细胞表面标志物表达、间充质谱系分化等方面无明显差异。然而,在0 ~ 1/3根吸收(G1)的牙齿中,SHED表现出相对较高的增殖能力和所选标志物的表达。综上所述,shed (G1)和shed (G2)表现出相似的细胞和生物学特性,使它们有可能应用于再生治疗。
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来源期刊
Stem cell investigation
Stem cell investigation Biochemistry, Genetics and Molecular Biology-Developmental Biology
CiteScore
5.80
自引率
0.00%
发文量
9
期刊介绍: The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.
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