Characterization of Acetylxylan Esterase from White-Rot Fungus Irpex lacteus.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of applied glycoscience Pub Date : 2019-10-24 eCollection Date: 2019-01-01 DOI:10.5458/jag.jag.JAG-2019_0007

Sangho Koh, Seika Imamura, Naoto Fujino, Masahiro Mizuno, Nobuaki Sato, Satoshi Makishima, Peter Biely, Yoshihiko Amano

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引用次数: 2

Abstract

The carbohydrate esterase family 1 (CE1) in CAZy contains acetylxylan esterases (AXEs) and feruloyl esterases (FAEs). Here we cloned a gene coding for an AXE belonging to CE1 from Irpex lacteus (IlAXE1). IlAXE1 was heterologously expressed in Pichia pastoris, and the recombinant enzyme was purified and characterized. IlAXE1 hydrolyzed p-nitrophenyl acetate, α-naphthyl acetate and 4-methylumbelliferyl acetate, however, it did not show any activity on ethyl ferulate and methyl p-coumarate. We also examined the activity on partially acetylated and feruloylated xylan extracted from corncob by hydrothermal reaction. Similarly, ferulic and p-coumaric acids were not liberated, and acetic acid was only detected in the reaction mixture. The results indicated that IlAXE1 is an acetylxylan esterase actually reacted to acetyl xylan. However, since IlAXE1 was unable to completely release acetic acid esterifying xylopyranosyl residues, it is assumed that acetyl groups exhibiting resistance to deacetylation by IlAXE1 are present in corn cob xylan.

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白腐菌乙酰木聚糖酯酶的研究。

CAZy的碳水化合物酯酶家族1 (CE1)包含乙酰木聚糖酯酶(AXEs)和阿铁酰酯酶(FAEs)。在此，我们从Irpex lacteus (IlAXE1)中克隆了一个属于CE1的AXE编码基因。在毕赤酵母中异种表达了IlAXE1，并对重组酶进行了纯化和鉴定。IlAXE1可水解对硝基苯乙酸酯、α-萘乙酸酯和4-甲基伞花乙酸酯，但对阿威酸乙酯和对香豆酸甲酯没有水解活性。研究了水热法提取玉米芯部分乙酰化木聚糖和阿魏烯化木聚糖的活性。同样，阿魏酸和对香豆酸也没有被释放，醋酸只在反应混合物中被检测到。结果表明IlAXE1是一种与乙酰木聚糖发生反应的乙酰木聚糖酯酶。然而，由于IlAXE1不能完全释放醋酸酯化xylopyranoyl残基，因此可以假设在玉米芯木聚糖中存在抵抗IlAXE1去乙酰化的乙酰基。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied glycoscience BIOCHEMISTRY & MOLECULAR BIOLOGY-

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9.10%

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