Effect of piceatannol against malignant melanoma in vivo and in vitro.

Q3 Medicine

华西口腔医学杂志 Pub Date : 2021-08-01 DOI:10.7518/hxkq.2021.04.006

Bo Yu, Wei Liu, Min-Qi Hu, Xiu-Fa Tang, Chun-Jie Li, Lin Que

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引用次数: 0

Abstract

Objectives: To study the antitumor effect of piceatannol (PIC) on malignant melanoma in vitro and in vivo.

Methods: B16F10 cells were cultured in vitro and treated with gradient concentrations of PIC. Cell viability was detected with methyl thiazolyl tetrazolium (MTT) assay; matrix metalloproteinase (MMP)-2, MMP-9, vascular endothelial growth factor (VEGF), spleen tyrosine kinase (Syk), and p-Syk were detected with Western blot; migration ability was detected with wound healing assay; invasion ability was detected with Transwell assay. Syk expression was suppressed through RNA interference for the detection of the possible mechanism of PIC in melanoma. An in vivo study was established by creating B16F10-bearing mice with intraperitoneal injection of PIC.

Results: The cell viability of B16F10 decreased with increasing PIC concentration. The results of the Transwell assay showed that invasion ability decreased with increasing PIC concentration, and healing time was prolonged at increased PIC concentration in the wound healing assay. Western blot results showed that PIC mainly inhibited the phosphorylation of Syk and inhibited the expression of MMP-2, MMP-9, and VEGF. RNA interference pointed out that blocking the expression of Syk can reveal the same inhibition effect on B16F10 cells as PIC. In vivo study revealed that different concentrations of PIC cangreatly inhibit melanoma progression.

Conclusions: PIC might block the progression of malignant melanoma by inhibiting spleen tyrosine kinase.

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皮夏单宁对体内和体外恶性黑色素瘤的影响

目的：研究皮夏单宁对恶性黑色素瘤的体外和体内抗肿瘤作用：研究皮脂单酚（PIC）在体外和体内对恶性黑色素瘤的抗肿瘤作用：方法：体外培养 B16F10 细胞并用梯度浓度的 PIC 处理。方法：体外培养 B16F10 细胞并用梯度浓度的 PIC 处理，用甲基噻唑基四氮唑（MTT）检测细胞活力；用 Western 印迹法检测基质金属蛋白酶（MMP）-2、MMP-9、血管内皮生长因子（VEGF）、脾酪氨酸激酶（Syk）和 p-Syk；用伤口愈合试验检测迁移能力；用 Transwell 试验检测侵袭能力。通过 RNA 干扰抑制 Syk 的表达，以检测 PIC 在黑色素瘤中的可能机制。通过腹腔注射 PIC，建立了 B16F10 小鼠的体内研究：结果：随着事先知情同意浓度的增加，B16F10 的细胞活力下降。Transwell 试验结果表明，随着 PIC 浓度的增加，细胞侵袭能力下降；在伤口愈合试验中，PIC 浓度增加时伤口愈合时间延长。Western 印迹结果显示，PIC 主要抑制 Syk 的磷酸化，并抑制 MMP-2、MMP-9 和 VEGF 的表达。RNA 干扰表明，阻断 Syk 的表达与 PIC 对 B16F10 细胞的抑制作用相同。体内研究表明，不同浓度的 PIC 能极大地抑制黑色素瘤的发展：结论：PIC可通过抑制脾脏酪氨酸激酶阻断恶性黑色素瘤的进展。

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来源期刊

华西口腔医学杂志 Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

6397

期刊介绍： West China Journal of Stomatology (WCJS, pISSN 1000-1182, eISSN 2618-0456, CN 51-1169/R), published bimonthly, is a peer-reviewed Open Access journal, hosted by Sichuan university and Ministry of Education of the People's Republic of China. WCJS was established in 1983 and indexed in Medline/Pubmed, SCOPUS, EBSCO, Chemical Abstract(CA), CNKI, WANFANG Data, etc.