A Simple Method to Establish Metaphase Chromosomes from Individual Zebrafish Embryos.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-10-01 Epub Date: 2021-08-11 DOI:10.1089/zeb.2021.0014

Halida Thanveer Asana Marican, Lucas W H Sun, Hongyuan Shen

引用次数: 1

Abstract

Cytogenetic approach based on metaphase chromosomes established from dividing cells enables direct microscopic visualization of individual chromosomes, a powerful technique to investigate aneuploidy, chromosome aberrations, and genomic instability. In this study, we describe a simple method based on direct blocking of metaphases in individual zebrafish embryo and dropping slides with temperature changes, water vapor, and acetic acid treatment to increase the metaphase diameters. We demonstrate that well-separated metaphases could be established from single zebrafish embryos using this method. Our method could be further adapted for the analyses of DNA damage, chromosome aberrations, and genomic instability using zebrafish and other teleost models.

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一种从斑马鱼个体胚胎中建立中期染色体的简单方法。

基于分裂细胞建立的中期染色体的细胞遗传学方法可以直接在显微镜下观察单个染色体，这是一种研究非整倍体、染色体畸变和基因组不稳定性的强大技术。在本研究中，我们描述了一种简单的方法，即直接阻断斑马鱼个体胚胎的中期，并在温度变化、水蒸气和醋酸处理的情况下滴载玻片，以增加中期直径。我们证明用这种方法可以从单个斑马鱼胚胎中建立分离良好的中期。我们的方法可以进一步适用于斑马鱼和其他硬骨鱼模型的DNA损伤、染色体畸变和基因组不稳定性分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.