A Simple Method to Establish Metaphase Chromosomes from Individual Zebrafish Embryos.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2021-10-01 Epub Date: 2021-08-11 DOI:10.1089/zeb.2021.0014

Halida Thanveer Asana Marican, Lucas W H Sun, Hongyuan Shen

引用次数: 1

Abstract

Cytogenetic approach based on metaphase chromosomes established from dividing cells enables direct microscopic visualization of individual chromosomes, a powerful technique to investigate aneuploidy, chromosome aberrations, and genomic instability. In this study, we describe a simple method based on direct blocking of metaphases in individual zebrafish embryo and dropping slides with temperature changes, water vapor, and acetic acid treatment to increase the metaphase diameters. We demonstrate that well-separated metaphases could be established from single zebrafish embryos using this method. Our method could be further adapted for the analyses of DNA damage, chromosome aberrations, and genomic instability using zebrafish and other teleost models.

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一种从斑马鱼个体胚胎中建立中期染色体的简单方法。

基于分裂细胞建立的中期染色体的细胞遗传学方法可以直接在显微镜下观察单个染色体，这是一种研究非整倍体、染色体畸变和基因组不稳定性的强大技术。在本研究中，我们描述了一种简单的方法，即直接阻断斑马鱼个体胚胎的中期，并在温度变化、水蒸气和醋酸处理的情况下滴载玻片，以增加中期直径。我们证明用这种方法可以从单个斑马鱼胚胎中建立分离良好的中期。我们的方法可以进一步适用于斑马鱼和其他硬骨鱼模型的DNA损伤、染色体畸变和基因组不稳定性分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.