Effects of Sex and Group Size on Behavior and Brain Biogenic Amines in Short-Lived Turquoise Killifish (Nothobranchius furzeri).

Abstract

Short-lived turquoise killifish (Nothobranchius furzeri) becomes a popular model species for neuroscience. However, the effects of sex and rearing conditions on behavior and brain monoamines in N. furzeri are unknown. In this article, we study the body mass, behavior in the novel tank diving test, levels of noradrenaline (NA), dopamine (DA), serotonin (5-HT), 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) in the brain of 108 day-old N. furzeri males and females reared in small (one male and one or two females in 4-L tanks) and large (four males and four females in 25-L tanks) groups. Males were heavier and had a lower NA level in the brain compared with females. The behavior of males and females did not differ in the novel tank diving test. Their DA, 5-HT, DOPAC, and 5-HIAA levels in the brain did not differ too. Males from small groups spent more time near the tank's bottom. Rearing in small groups reduced the DA level in the female brain and the DOPAC level in female and male brains. However, group size did not affect body mass, 5-HT and 5-HIAA levels in the brain. Thus, group size is important for behavior and neuroscience studies of N. furzeri.