Karyotypic stability of Fragaria (strawberry) species revealed by cross-species chromosome painting.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2021-12-01 Epub Date: 2021-06-21 DOI:10.1007/s10577-021-09666-1
Manman Qu, Luyue Zhang, Kunpeng Li, Jianying Sun, Zongyun Li, Yonghua Han
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引用次数: 7

Abstract

Chromosome karyotyping analysis is particularly useful in determining species relationships and the origin of polyploid species. Identification of individual chromosomes is the foundation for karyotype development. For Fragaria (strawberry) species, definitive identification of the individual chromosomes is extremely difficult because of their small size and similar shape. Here, we identified all chromosomes for 11 representative Fragaria species with different ploidy using a set of oligonucleotide-based probes developed in Fragaria vesca. Comprehensive molecular cytogenetic karyotypes were established based on the individually identified chromosomes. In addition, we used oligo probes to assign the 5S and 45S rDNA loci to specific chromosomes in 16 Fragaria species. We found that these Fragaria species maintained a remarkably conserved karyotype. No inter-chromosomal structural rearrangements at the cytological level were observed in any of the chromosomes among these species. Despite karyotypic stability and similarity, variations in the signal intensity of oligo probes were observed among the homologous chromosomes in several polyploid species. Moreover, most Fragaria species also showed differences in the distribution patterns of 45S and 5S rDNA. These data provide new insights into the origins of several polyploid Fragaria species.

跨种染色体绘制揭示草莓属植物核型稳定性。
染色体核型分析在确定物种关系和多倍体物种的起源方面特别有用。单个染色体的鉴定是核型发育的基础。对于草莓(草莓)物种,个体染色体的明确鉴定是极其困难的,因为它们的小尺寸和相似的形状。在此,我们使用一套基于寡核苷酸的探针,鉴定了11种具有不同倍性的代表性的Fragaria vesca物种的所有染色体。根据单个鉴定的染色体建立了全面的分子细胞遗传学核型。此外,我们使用寡核苷酸探针在16个Fragaria种的特定染色体上定位了5S和45S rDNA位点。我们发现这些Fragaria物种保持了一个非常保守的核型。在这些物种中,没有观察到任何染色体在细胞学水平上的染色体间结构重排。尽管核型稳定性和相似性,但在几种多倍体物种的同源染色体中观察到寡核苷酸探针的信号强度存在差异。此外,大多数Fragaria种在45S和5S rDNA的分布模式上也存在差异。这些数据为一些多倍体Fragaria物种的起源提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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