Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

IF 2.6 Q3 IMMUNOLOGY
International Journal of Inflammation Pub Date : 2021-05-26 eCollection Date: 2021-01-01 DOI:10.1155/2021/6695468
Vadim Genkel, Ilya Dolgushin, Irina Baturina, Albina Savochkina, Alla Kuznetsova, Lubov Pykhova, Igor Shaposhnik
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引用次数: 3

Abstract

Background: There is strong evidence to suggest that the negative influence of triglyceride-rich lipoproteins (TRLs) on atherosclerosis development and progression is at least partially mediated by their proinflammatory effects. However, the effect of hypertriglyceridemia (HTG) on the subpopulation composition of circulating neutrophils has not been studied so far. The aim of this study was to examine correlations between the level of triglycerides (TGs) and the subpopulation composition of circulating neutrophils in middle-aged patients with dyslipidemia without established atherosclerotic cardiovascular diseases (ASCVDs).

Methods: Ninety-one patients with dyslipidemia, including 22 (24.2%) patients with HTG, were enrolled in the study. Phenotying of neutrophil subpopulations was performed through flow cytometry (Navios 6/2, Beckman Coulter, USA). For phenotyping of neutrophil subpopulations, conjugated monoclonal antibodies were used: CD16, PE-Cyanine7 (Invitrogen, USA); CD11b-FITC (Beckman Coulter, USA); CD62L-PE (Beckman Coulter, USA); and CD184 (CXCR4)-PE-CF594 (BD Biosciences, USA).

Results: Following the correlation analysis, the TG level directly correlated with the number of circulating leukocytes (r = 0.443; p < 0.0001) and neutrophils (r = 0.311; p=0.008). HTG patients displayed a significantly high number of circulating neutrophils with CD16hiCD11bhiCD62Lhi and CD16hiCD11bloCD62Lbr phenotypes. TG levels directly correlated with the number of circulating neutrophils having CD16hiCD11bhiCD62Lhi and CD16hiCD11bloCD62Lbr phenotypes. Following the linear regression analysis, statistically significant correlations between TG levels and neutrophil subpopulations having CD16hiCD11bloCD62Lbr and CD16hiCD11bbrCD62LloCXCR4hi phenotypes were established. Changes in TG levels could explain up to 19.1% of the variability in the number of studied neutrophil subpopulations.

Conclusion: Among middle-aged patients without established ASCVDs, patients with HTG demonstrated a significantly higher overall number of neutrophils and neutrophils having CD16hiCD11bhiCD62Lhi (mature neutrophils) and CD16hiCD11bloCD62Lbr (immunosuppressive neutrophils) than patients with normal TG levels. The TG level was associated with an increase in the number of CD16hiCD11bloCD62Lbr and CD16hiCD11bbrCD62LloCXCR4hi (ageing neutrophils) neutrophils, adjusted for the sex and age of the patients.

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血脂异常患者高甘油三酯血症与循环中性粒细胞亚群之间的关系
背景:有强有力的证据表明,富甘油三酯脂蛋白(TRLs)对动脉粥样硬化发生和进展的负面影响至少部分是由其促炎作用介导的。然而,到目前为止,高甘油三酯血症(HTG)对循环中性粒细胞亚群组成的影响尚未得到研究。本研究的目的是研究未确诊动脉粥样硬化性心血管疾病(ascvd)的中年血脂异常患者的甘油三酯(tg)水平与循环中性粒细胞亚群组成之间的相关性。方法:纳入91例血脂异常患者,其中HTG患者22例(24.2%)。中性粒细胞亚群的表型通过流式细胞术进行(Navios 6/2, Beckman Coulter, USA)。对于中性粒细胞亚群的表型,使用偶联单克隆抗体:CD16, PE-Cyanine7 (Invitrogen, USA);CD11b-FITC (Beckman Coulter, USA);CD62L-PE (Beckman Coulter, USA);CD184 (CXCR4)-PE-CF594 (BD Biosciences, USA)。结果:经相关分析,TG水平与循环白细胞数直接相关(r = 0.443;P < 0.0001)和中性粒细胞(r = 0.311;p = 0.008)。HTG患者表现出CD16hiCD11bhiCD62Lhi和CD16hiCD11bloCD62Lbr表型的大量循环中性粒细胞。TG水平与CD16hiCD11bhiCD62Lhi和CD16hiCD11bloCD62Lbr表型的循环中性粒细胞数量直接相关。经线性回归分析,TG水平与CD16hiCD11bloCD62Lbr和CD16hiCD11bbrCD62LloCXCR4hi表型的中性粒细胞亚群之间存在统计学意义的相关性。TG水平的变化可以解释所研究的中性粒细胞亚群数量变异的19.1%。结论:在未确诊ascvd的中年患者中,HTG患者的中性粒细胞总数和CD16hiCD11bhiCD62Lhi(成熟中性粒细胞)和CD16hiCD11bloCD62Lbr(免疫抑制中性粒细胞)的中性粒细胞总数明显高于正常TG水平的患者。TG水平与CD16hiCD11bloCD62Lbr和CD16hiCD11bbrCD62LloCXCR4hi(衰老中性粒细胞)中性粒细胞数量的增加有关,根据患者的性别和年龄进行调整。
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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
16
审稿时长
16 weeks
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