Twenty-four hour ex-vivo normothermic machine perfusion in rat livers.

TECHNOLOGY Pub Date : 2020-03-01 Epub Date: 2020-11-05 DOI:10.1142/s2339547820500028
Omar Haque, Casie A Pendexter, Stephanie E J Cronin, Siavash Raigani, Reiner J de Vries, Heidi Yeh, James F Markmann, Korkut Uygun
{"title":"Twenty-four hour ex-vivo normothermic machine perfusion in rat livers.","authors":"Omar Haque, Casie A Pendexter, Stephanie E J Cronin, Siavash Raigani, Reiner J de Vries, Heidi Yeh, James F Markmann, Korkut Uygun","doi":"10.1142/s2339547820500028","DOIUrl":null,"url":null,"abstract":"<p><p>Ex-vivo liver perfusion (EVLP) is an ideal platform to study liver disease, therapeutic interventions, and pharmacokinetic properties of drugs without any patient risk. Rat livers are an ideal model for EVLP due to less organ quality variability, ease of hepatectomy, well-defined molecular pathways, and relatively low costs compared to large animal or human perfusions. However, the major limitation with rat liver normothermic machine perfusion (NMP) is maintaining physiologic liver function on an ex-vivo machine perfusion system. To address this need, our research demonstrates 24-hour EVLP in rats under normothermic conditions. Early (6 hour) perfusate transaminase levels and oxygen consumption of the liver graft are shown to be good markers of perfusion success and correlate with viable 24-hour post-perfusion histology. Finally, we address overcoming challenges in long-term rat liver perfusions such as rising intrahepatic pressures and contamination, and offer future directions necessary to build upon our work.</p>","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"8","pages":"27-36"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300916/pdf/nihms-1646032.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"TECHNOLOGY","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1142/s2339547820500028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/11/5 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Ex-vivo liver perfusion (EVLP) is an ideal platform to study liver disease, therapeutic interventions, and pharmacokinetic properties of drugs without any patient risk. Rat livers are an ideal model for EVLP due to less organ quality variability, ease of hepatectomy, well-defined molecular pathways, and relatively low costs compared to large animal or human perfusions. However, the major limitation with rat liver normothermic machine perfusion (NMP) is maintaining physiologic liver function on an ex-vivo machine perfusion system. To address this need, our research demonstrates 24-hour EVLP in rats under normothermic conditions. Early (6 hour) perfusate transaminase levels and oxygen consumption of the liver graft are shown to be good markers of perfusion success and correlate with viable 24-hour post-perfusion histology. Finally, we address overcoming challenges in long-term rat liver perfusions such as rising intrahepatic pressures and contamination, and offer future directions necessary to build upon our work.

对大鼠肝脏进行 24 小时体外常温机器灌注。
体外肝脏灌注(EVLP)是研究肝脏疾病、治疗干预和药物药代动力学特性的理想平台,对病人没有任何风险。与大型动物或人体灌流相比,大鼠肝脏是EVLP的理想模型,因为大鼠肝脏的器官质量变异较小,易于肝切除,分子通路明确,而且成本相对较低。然而,大鼠肝脏常温机器灌注(NMP)的主要局限是在体外机器灌注系统上维持肝脏的生理功能。为了满足这一需求,我们的研究在常温条件下对大鼠进行了 24 小时 EVLP。早期(6 小时)灌注液转氨酶水平和肝脏移植物的耗氧量被证明是灌注成功的良好标志,并与灌注后 24 小时存活的组织学相关。最后,我们讨论了如何克服长期大鼠肝脏灌注过程中遇到的挑战,如肝内压力升高和污染等,并提出了在我们的工作基础上继续前进的必要方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
TECHNOLOGY
TECHNOLOGY ENGINEERING, MULTIDISCIPLINARY-
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信