Establishment of a diabetes susceptible family in the Bama minipig (BMP) by N-ethyl-N-nitrosourea (ENU) induction.

IF 0.6 4区医学 Q4 ENDOCRINOLOGY & METABOLISM

Neuro endocrinology letters Pub Date : 2021-07-01

Xiaohua Su, Zhidong Li, Bayaer Nashun, Juncheng Deng, Min Yue, Ming Lv, Weiwang Gu

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引用次数: 0

Abstract

Background: Diabetes mellitus (DM), characterized by hyperglycemia, is one of the leading causes of death worldwide. An appropriate DM animal model to explore the underlying mechanism of DM and develop new antidiabetic drugs is still desirable. Here, we aim to provide alternatives of DM animal models for medical researches.

Objective: To establish a diabetes susceptible family in the Bama minipig (BMP) by N-ethyl-N-nitrosourea (ENU) induction.

Methods: Male BMPs with hyperglycemia were selected from G1 and bred by the inbreeding strategy. After 5 generations, parameters such as fasting plasma glucose (FPG), 2-hour plasma glucose (2hPG), intravenous glucose-tolerance test (IVGTT), and insulin resistance were determined to evaluate susceptible family members.

Results: The male BMP 2907 (FPG = 6.1 mmol/L, IGVTT 2hPG = 11.9 mmol/L) with hyperglycemia was selected from G1 to generate the 2907 hyperglycemic family. With the number of breeding generations, average FPG levels in BMPs increased significantly (p < 0.05). G5 displayed the characteristics of elevated FPG, insulin resistance, dyslipidemia and abnormal glucose tolerance (p < 0.05).

Conclusion: A diabetes susceptible family has been successfully established, which might be used for further inbreeding or induced to mimic the phenotype of diabetes.

本刊更多论文

用n -乙基-n -亚硝基脲(ENU)诱导建立巴马迷你猪糖尿病易感家族。

背景:糖尿病(DM)以高血糖为特征，是世界范围内死亡的主要原因之一。建立合适的糖尿病动物模型，以探索糖尿病的发病机制和开发新的抗糖尿病药物是十分必要的。在此，我们旨在为医学研究提供DM动物模型的替代方案。目的:通过n -乙基-n -亚硝基脲(ENU)诱导建立巴马迷你猪(BMP)糖尿病易感家族。方法:从G1组中选择高血糖雄性BMPs，采用近交策略选育。5代后，测定空腹血糖(FPG)、2小时血糖(2hPG)、静脉葡萄糖耐量试验(IVGTT)、胰岛素抵抗等参数，评价易感家族成员。结果:从G1中选取高血糖男性BMP 2907 (FPG = 6.1 mmol/L, IGVTT 2hPG = 11.9 mmol/L)形成2907高血糖家族。随着繁殖代数的增加，bmp中FPG的平均水平显著升高(p < 0.05)。G5表现为FPG升高、胰岛素抵抗、血脂异常、糖耐量异常(p < 0.05)。结论:成功建立了一个糖尿病易感家族，该家族可用于进一步近亲繁殖或诱导模仿糖尿病表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuro endocrinology letters 医学-内分泌学与代谢

CiteScore

1.00

自引率

14.30%

发文量

审稿时长

6 months

期刊介绍： Neuroendocrinology Letters is an international, peer-reviewed interdisciplinary journal covering the fields of Neuroendocrinology, Neuroscience, Neurophysiology, Neuropsychopharmacology, Psychoneuroimmunology, Reproductive Medicine, Chronobiology, Human Ethology and related fields for RAPID publication of Original Papers, Review Articles, State-of-the-art, Clinical Reports and other contributions from all the fields covered by Neuroendocrinology Letters. Papers from both basic research (methodology, molecular and cellular biology, anatomy, histology, biology, embryology, teratology, normal and pathological physiology, biophysics, pharmacology, pathology and experimental pathology, biochemistry, neurochemistry, enzymology, chronobiology, receptor studies, endocrinology, immunology and neuroimmunology, animal physiology, animal breeding and ethology, human ethology, psychology and others) and from clinical research (neurology, psychiatry and child psychiatry, obstetrics and gynecology, pediatrics, endocrinology, immunology, cardiovascular studies, internal medicine, oncology and others) will be considered. The Journal publishes Original papers and Review Articles. Brief reports, Special Communications, proved they are based on adequate experimental evidence, Clinical Studies, Case Reports, Commentaries, Discussions, Letters to the Editor (correspondence column), Book Reviews, Congress Reports and other categories of articles (philosophy, art, social issues, medical and health policies, biomedical history, etc.) will be taken under consideration.