Immune Responses in Cutaneous Leishmaniasis: In vitro Thelper1/Thelper2 Cytokine Profiles Using Live Versus Killed Leishmania major.

IF 0.6 4区医学 Q4 PARASITOLOGY

Journal of Arthropod-Borne Diseases Pub Date : 2021-03-31 eCollection Date: 2021-03-01 DOI:10.18502/jad.v15i1.6491

Akram Miramin-Mohammadi, Amir Javadi, Seyyed Ebrahim Eskandari, Mahmood Nateghi-Rostami, Ali Khamesipour

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引用次数: 2

Abstract

Background: Recovery from cutaneous leishmaniasis (CL) leads to protection against further lesion development. In contrast, vaccination using killed parasites does not induce enough protection; the reason(s) is not currently known but might be related to different immune response induced against live versus killed parasites. In this study, Th1/Th2 cytokine profiles of CL patients were evaluated against live versus killed Leishmania major.

Methods: In this study peripheral blood mononuclear cells (PBMC) of the volunteers with active CL lesion (CL), history of CL (HCL) and healthy volunteers were cultured and stimulated with live or killed Leishmania major, the supernatants were collected and levels of IFN-γ, IL-5 and IL-10 were titrated using ELISA method.

Results: The results showed that IFN-γ levels in CL patients (p< 0.001) and HCL volunteers (p< 0.005) are significantly higher when stimulated with live than stimulated with killed L. major. IFN-γ production in PBMC volunteers with CL and HCL stimulated with live or heat-killed L. major was significantly (p< 0.001) higher than in unstimulated ones. The level of IL-5 in CL patients (p< 0.005) and HCL volunteers (p< 0.001) are significantly lower when stimulated with live than killed L. major. There was no significant difference between the levels of IL-10 in PBMC stimulated with either live or killed L. major.

Conclusion: It is concluded that using live Leishmania induces a stronger Th1 type of immune response which justify using leishmanization as a control measure against CL.

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皮肤利什曼病的免疫反应:活利什曼病与死利什曼病的体外细胞因子分析

背景:皮肤利什曼病(CL)的恢复可以防止进一步的病变发展。相反，使用被杀死的寄生虫接种疫苗不能产生足够的保护;其原因目前尚不清楚，但可能与对活寄生虫和死寄生虫诱导的不同免疫反应有关。在这项研究中，对CL患者的Th1/Th2细胞因子谱进行了活的和死的利什曼原虫的评估。方法:采用活的或灭活的利什曼原虫刺激有活动CL病变(CL)、有CL病史(HCL)和健康志愿者外周血单个核细胞(PBMC)，收集上清液，采用ELISA法测定IFN-γ、IL-5和IL-10水平。结果:CL患者(p< 0.001)和HCL志愿者(p< 0.005)在活刺激下IFN-γ水平明显高于灭活刺激。活的或热灭的L. major刺激CL和HCL的PBMC志愿者IFN-γ的产生显著高于未刺激的志愿者(p< 0.001)。CL患者(p< 0.005)和HCL志愿者(p< 0.001)在活体刺激下IL-5水平明显低于杀死L. major。IL-10在大白介素活生和死生刺激下均无显著差异。结论:利什曼原虫活株可诱导较强的Th1型免疫反应，证明采用利什曼原虫活株作为防治措施是合理的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

8 weeks

期刊介绍： The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.