Molecular Identification and Genotyping of Babesia canis in Dogs from Meshkin Shahr County, Northwestern Iran.

IF 0.6 4区 医学 Q4 PARASITOLOGY
Journal of Arthropod-Borne Diseases Pub Date : 2021-03-31 eCollection Date: 2021-03-01 DOI:10.18502/jad.v15i1.6489
Majid Khanmohammadi, Reza Zolfaghari-Emameh, Mehdi Arshadi, Elham Razmjou, Poorya Karimi
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引用次数: 4

Abstract

Background: Canine babesiosis is one of the mainly worldwide-distributed tick-borne haemoprotozoan parasitic diseases in dogs.

Methods: A total of 43 blood samples were randomly collected from naturally infected dogs in seven villages from different geographical areas of Meshkin Shahr, Ardabil Province, Iran. The presence of Babesia species detected with standard methods including parasitological and gene sequencing techniques targeting the 18S rRNA gene.

Results: Our results revealed that four dogs 9.3% (4/43) including one female and three male dogs were infected with Babesia. All four Babesia-infected dogs were confirmed B. canis by the molecular-based method. Sequence alignments comparison of the B. canis genotypes A and B, it was revealed that all B. canis isolates belonged to genotype B.

Conclusion: This study provides essential data for subsequently define the critical importance of the molecular studies in management and prevention of the canine babesiosis in Iran.

Abstract Image

Abstract Image

Abstract Image

伊朗西北部Meshkin Shahr县犬巴贝斯虫的分子鉴定和基因分型。
背景:犬巴贝斯虫病是一种主要分布在世界范围内的蜱传犬寄生虫病。方法:在伊朗阿达比勒省Meshkin Shahr不同地理区域的7个村庄随机采集自然感染犬43份血样。采用寄生虫学和18S rRNA基因测序等标准方法检测巴贝斯虫。结果:4只犬(9.3%)感染巴贝斯虫,其中1只母犬和3只公犬感染巴贝斯虫。4只感染巴贝斯虫的犬均经分子检测证实为犬巴贝斯虫。通过对犬巴贝斯虫基因型A和基因型B的序列比对,发现所有犬巴贝斯虫分离株均属于基因型B。结论:本研究为进一步明确分子研究在伊朗犬巴贝斯虫病防治中的重要意义提供了必要的数据。
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来源期刊
Journal of Arthropod-Borne Diseases
Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY
CiteScore
2.00
自引率
11.10%
发文量
17
审稿时长
8 weeks
期刊介绍: The journal publishes original research paper, short communica­tion, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases includ­ing: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.
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