Altered expression of specific antioxidant (SOD1 and SOD2) and DNA repair (XRCC1 and OGG1) genes in patients with newly diagnosed type-2 diabetes mellitus.

IF 2.5 Q3 ENDOCRINOLOGY & METABOLISM
Minerva endocrinology Pub Date : 2024-12-01 Epub Date: 2021-06-23 DOI:10.23736/S2724-6507.21.03417-5
Smriti Suri, Prasenjit Mitra, Abhilasha Abhilasha, Indu Saxena, M K Garg, Gopal K Bohra, Praveen Sharma
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引用次数: 0

Abstract

Background: Uncontrolled increase in reactive oxygen species (ROS) leads to the release of free radicals. Additionally, when antioxidants go below a certain level, major molecules of our system such as DNA, proteins, and many other macromolecules get damaged, leading to cancer, heart diseases, and metabolic syndromes like diabetes. Therefore, in our study we focused on: 1) newly diagnosed type 2 diabetes mellitus (T2DM) patients and tried to evaluate the expression of antioxidant enzyme encoding genes; 2) superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2) and DNA repair genes; and 3) X-ray repair cross-complementing 1(XRCC1) and 8-oxoguanine DNA glycosylase 1 (OGG1) in them.

Methods: Expression analysis was performed by RT-PCR on 60 subjects (30 T2DM cases and 30 non-diabetic controls). The level of the SOD enzyme was also estimated in a serum sample by the colorimetric method. Biochemical parameters such as fasting plasma glucose (FBG), glycated haemoglobin (HbA1c), high sensitivity C-reactive protein (hsCRP), and lipid profile were estimated in an auto analyzer. Receiver operating characteristic (ROC) curve analysis was done, the area under the curve for mRNA expression and enzyme level was calculated to determine their potential as markers in newly diagnosed T2DM.

Results: Down-regulation of both SOD1 (0.43 fold, P=0.02) and SOD2 (0.41 fold, P=0.13) and up-regulation of both XRCC1 (1.15 fold, P>0.05) and OGG1 (1.49 fold, P>0.05) was observed in patients with T2DM. We also observed a significant decrease (P=0.02) in SOD enzyme levels in diabetic cases than in controls (599.8±178.9 and 691.3±127.3).

Conclusions: We report that antioxidant repair genes are downregulated and DNA repair genes are upregulated in newly diagnosed T2DM patients. SOD levels and SOD1 gene expression can serve as informative biomarkers for identifying T2DM patients.

新诊断出的 2 型糖尿病患者体内特定抗氧化基因(SOD1 和 SOD2)和 DNA 修复基因(XRCC1 和 OGG1)的表达发生变化。
背景:活性氧(ROS)的无节制增加会导致自由基的释放。此外,当抗氧化剂低于一定水平时,我们系统中的主要分子(如 DNA、蛋白质和许多其他大分子)就会受损,从而导致癌症、心脏病和糖尿病等代谢综合征。因此,我们以新诊断的 2 型糖尿病(T2DM)患者为研究对象,试图评估他们体内抗氧化酶编码基因--超氧化物歧化酶 1(SOD1)和超氧化物歧化酶 2(SOD2)以及 DNA 修复基因--X 射线修复交叉互补 1(XRCC1)和 8-氧代鸟嘌呤 DNA 糖基化酶 1(OGG1)的表达情况:对 60 名受试者(30 名 T2DM 病例和 30 名非糖尿病对照组)进行了 RT-PCR 表达分析。此外,还采用比色法估测了血清样本中 SOD 酶的水平。生化参数,如空腹血浆葡萄糖 (FBG)、糖化血红蛋白 (HbA1c)、高敏 C 反应蛋白 (hsCRP) 和血脂概况均通过自动分析仪进行估算。进行了接收者操作特征曲线(ROC)分析,计算了 mRNA 表达和酶水平的曲线下面积,以确定它们作为新诊断 T2DM 标志物的潜力:结果:在 T2DM 患者中观察到 SOD1(0.43 倍,p=0.02)和 SOD2(0.41 倍,p=0.13)下调,XRCC1(1.15 倍,p>0.05)和 OGG1(1.49 倍,p>0.05)上调。我们还观察到,糖尿病病例的 SOD 酶水平(599.8 ± 178.9 和 691.3 ± 127.3)明显低于对照组(p=0.02):我们发现,在新诊断的 T2DM 患者中,抗氧化修复基因下调,而 DNA 修复基因上调。SOD水平和SOD1基因表达可作为识别T2DM患者的信息生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
146
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