Increased Estradiol Levels Effect on Leptin Expression in Midluteal Endometrial Tissue of Macaca Nemestrina (Southern Pig-Tailed Macaques).

Journal of Family and Reproductive Health Pub Date : 2020-12-01 DOI:10.18502/jfrh.v14i4.5206

Nurhuda Sahar, Adriana Viola Miranda, Afif Rasyad, Karina Rahmaningrum, Kusmardi Kusmardi, Diyah Kristanty

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Abstract

Objective: To measure the effect of serum estradiol (E2) levels on leptin expression in Macaca nemestrina (southern pig-tailed macaque) endometrial tissue. Materials and methods: This study used paraffin-embedded midluteal phase endometrial tissue blocks of Macaca nemestrina from previous study. Included subjects were 15 female macaques of reproductive age (8-10 years) with a previous history of producing offspring, which were divided into four groups: groups administered with 30 IU, 50 IU, and 70 IU r-FSH (intervention group), and no r-FSH (control group). The stimulation was done following GnRH agonist long protocol. Staining was done using immunohistochemistry. Leptin expression was measured using immunohistochemistry (IHC) Profiler plugin of ImageJ software and counted semi-quantitatively as Histological Score (Hscore). Results: Correlation between E2 concentration to stromal leptin expression was observed (p=0.043). Conclusion: Serum estradiol concentration is found to be correlated with leptin expression in Macaca nemestrina, suggesting a mechanism of decreasing endometrial receptivity among women undergoing controlled ovarian hyperstimulation.

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雌二醇水平升高对南方猪尾猕猴黄体中部子宫内膜瘦素表达的影响。

目的:测定血清雌二醇(E2)水平对nemestrina (southern pig-tailed猕猴)子宫内膜瘦素表达的影响。材料与方法:本研究采用前人研究的猕猴黄体中期子宫内膜组织块石蜡包埋。研究对象为15只育龄(8-10岁)有生育史的雌性猕猴，分为4组:分别给予30 IU、50 IU和70 IU的促卵泡刺激素组(干预组)和不给予促卵泡刺激素组(对照组)。刺激是按照GnRH激动剂长期方案进行的。免疫组织化学染色。使用ImageJ软件的免疫组化(IHC) Profiler插件检测Leptin的表达，并半定量计算为组织学评分(Hscore)。结果:E2浓度与间质瘦素表达呈正相关(p=0.043)。结论:在猕猴体内，血清雌二醇浓度与瘦素表达相关，提示在控制性卵巢过度刺激的女性中存在降低子宫内膜容受性的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Family and Reproductive Health

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审稿时长

5 weeks

期刊介绍： The Journal of Family & Reproductive Health (JFRH) is the quarterly official journal of Vali–e–Asr Reproductive Health Research Center. This journal features fulllength, peerreviewed papers reporting original research, clinical case histories, review articles, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology including genetics, endocrinology, andrology, embryology, gynecologic urology, fetomaternal medicine, oncology, infectious disease, public health, nutrition, surgery, menopause, family planning, infertility, psychiatry–psychology, demographic modeling, perinatalogy–neonatolgy ethics and social issues, and pharmacotherapy. A high scientific and editorial standard is maintained throughout the journal along with a regular rate of publication. All published articles will become the property of the JFRH. The editor and publisher accept no responsibility for the statements expressed by the authors here in. Also they do not guarantee, warrant or endorse any product or service advertised in the journal.