Evolutionary divergence of motifs in B-class MADS-box proteins of seed plants.

IF 1.9 3区 生物学 Q2 BIOLOGY
Gangxu Shen, Yong Jia, Wei-Lung Wang
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引用次数: 3

Abstract

Background: MADS-box transcription factors function as homo- or heterodimers and regulate many aspects of plant development; moreover, MADS-box genes have undergone extensive duplication and divergence. For example, the morphological diversity of floral organs is closely related to the functional divergence of the MADS-box gene family. B-class genes (such as Arabidopsis thaliana APETALA3 [AP3] and PISTILLATA [PI]) belong to a subgroup of MADS-box genes. Here, we collected 97 MADS-box B protein sequences from 21 seed plant species and examined their motifs to better understand the functional evolution of B proteins.

Results: We used the MEME tool to identify conserved sequence motifs in these B proteins; unique motif arrangements and sequences were identified in these B proteins. The keratin-like domains of Malus domestica and Populus trichocarpa B proteins differed from those in other angiosperms, suggesting that a novel regulatory network might have evolved in these species. The MADS domains of Nelumbo nucifera, Glycine max, and Amborella trichopoda B-proteins contained motif 9; in contrast, those of other plants contained motif 1. Protein modelling analyses revealed that MADS domains with motif 9 may lack amino acid sites required for DNA-binding. These results suggested that the three species might share an alternative mechanism controlling floral development.

Conclusions: Amborella trichopoda has B proteins with either motif 1 or motif 9 MADS domains, suggesting that these two types of MADS domains evolved from the ancestral domain into two groups, those with motif 9 (N. nucifera and G. max), and those with motif 1. Moreover, our results suggest that the homodimer/heterodimer intermediate transition structure first appeared in A. trichopoda. Therefore, our systematic analysis of the motifs in B proteins sheds light on the evolution of these important transcription factors.

Abstract Image

Abstract Image

Abstract Image

种子植物b类MADS-box蛋白基序的进化分化。
背景:MADS-box转录因子作为同源或异源二聚体,调控植物发育的许多方面;此外,MADS-box基因经历了广泛的复制和分化。例如,花器官的形态多样性与MADS-box基因家族的功能分化密切相关。b类基因(如拟南芥APETALA3 [AP3]和PISTILLATA [PI])属于MADS-box基因的一个亚群。本文收集了21种种子植物的97个MADS-box B蛋白序列,并对其基序进行了分析,以期更好地了解B蛋白的功能进化。结果:我们使用MEME工具鉴定了这些B蛋白的保守序列基序;在这些B蛋白中鉴定出独特的基序排列和序列。家苹果和毛杨B蛋白的角蛋白样结构域与其他被子植物不同,这表明这些物种可能进化出一种新的调控网络。莲叶、甘氨酸和三叶Amborella trichopoda b蛋白的MADS结构域含有motif 9;相比之下,其他植物则含有motif 1。蛋白质模型分析显示,含有motif 9的MADS结构域可能缺乏dna结合所需的氨基酸位点。这些结果表明,这三个物种可能有一个共同的控制花发育的机制。结论:毛线虫B蛋白具有motif 1或motif 9的MADS结构域,表明这两种类型的MADS结构域从祖先结构域进化为motif 9 (N. nucifera和G. max)和motif 1。此外,我们的研究结果表明,同源二聚体/异源二聚体中间过渡结构最早出现在赤棘蟹中。因此,我们对B蛋白中基序的系统分析揭示了这些重要转录因子的进化。
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来源期刊
CiteScore
5.20
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.
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