Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-08-01 Epub Date: 2021-05-24 DOI:10.1089/zeb.2021.0006

Amanda Lipsitt, Nicole R Hensch, Rodrigo Moreno-Campos, Kunal Baxi, Jiangfei Chen, Anil K Challa, Eleanor Y Chen, Myron S Ignatius

引用次数: 1

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53^del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

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斑马鱼肿瘤移植物移植在体内生长肿瘤，移植物作为单细胞悬浮液效果不佳。

血管肉瘤是一种临床侵袭性肿瘤，死亡率高。它可以出现在血管或淋巴组织，涉及身体的任何部位，并积极地局部扩散或转移。血管肉瘤在tp53缺失(tp53del/del)斑马鱼突变体中自发发生。然而，血管肉瘤肿瘤的单细胞悬浮液的肿瘤解剖和移植的既定方案导致较低的植入率。为了解决这些并发症，我们开发了一种新的肿瘤移植技术，用于血管肉瘤和类似肿瘤在斑马鱼体内的植入，该技术维持了肿瘤微环境，具有优越的植入率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.