Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2021-08-01 Epub Date: 2021-05-24 DOI:10.1089/zeb.2021.0006

Amanda Lipsitt, Nicole R Hensch, Rodrigo Moreno-Campos, Kunal Baxi, Jiangfei Chen, Anil K Challa, Eleanor Y Chen, Myron S Ignatius

引用次数: 1

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53^del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

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斑马鱼肿瘤移植物移植在体内生长肿瘤，移植物作为单细胞悬浮液效果不佳。

血管肉瘤是一种临床侵袭性肿瘤，死亡率高。它可以出现在血管或淋巴组织，涉及身体的任何部位，并积极地局部扩散或转移。血管肉瘤在tp53缺失(tp53del/del)斑马鱼突变体中自发发生。然而，血管肉瘤肿瘤的单细胞悬浮液的肿瘤解剖和移植的既定方案导致较低的植入率。为了解决这些并发症，我们开发了一种新的肿瘤移植技术，用于血管肉瘤和类似肿瘤在斑马鱼体内的植入，该技术维持了肿瘤微环境，具有优越的植入率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.