Vitamin E and selenium improve mesenchymal stem cell conditioned media immunomodulatory effects.

Q1 Biochemistry, Genetics and Molecular Biology

Stem cell investigation Pub Date : 2021-05-07 eCollection Date: 2021-01-01 DOI:10.21037/sci-2020-008

Fereshteh Ghasemi, Majid Khoshmirsafa, Elahe Safari, Marzieh Asgari, Mehdi Alemrajabi, Shahrzad Nojehdehi, Samane Khorrami

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引用次数: 3

Abstract

Background: Mesenchymal stem cells (MSCs) with immunoregulatory properties affect immune systems. Many studies showed that antioxidants such as vitamin E (Vit E) and selenium (Se) could improve stem cells survival. This study aims to investigate the effects of MSC conditioned media (CM) treated with Vit E and Se on immune cells.

Methods: MSCs were isolated and cultured with Vit E and Se. Immature dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were cultured with MSC CM treated with Vit E and Se. The expression of HLA-DR, CD86, CD40, and CD83 on mature DC were evaluated. DC supernatant and PBMCs supernatant was collected for the study of TGF-β, IL-10, and IL-12. PBMCs evaluated for the expression of T-bet, GATA3, RORγt, and FOXP3.

Results: MSC CM increased CD40 on myeloid DC (mDC). CD40 has been decreased in DC treated with MSC (Vit E) and MSC (Se) CM. HLA-DR expression on DCs and IL-12 level were significantly reduced in MSC (Vit E) CM. IL-10 concentration increased in DCs treated with MSC (Vit E) and MSC (Se) CM. Treatment of PBMCs with MSC CM decreased IL-10 level, FOXP3, and RORγt expression. On the other hand, the MSC (Vit E) CM and MSC (Se) CM decreased the IL-10 level and increased IL-12, T-bet, and RORγt.

Conclusions: According to the results, the treatment of MSC with Vit E and Se enhanced the ability of MSCs to inhibit DCs and improved immunomodulatory effects. Concerning the effect of MSC on PBMC, it seems that it increased RORγt expression through monocytes.

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维生素E和硒提高间充质干细胞条件介质的免疫调节作用。

背景:间充质干细胞(MSCs)具有免疫调节特性，影响免疫系统。许多研究表明，抗氧化剂如维生素E (Vit E)和硒(Se)可以提高干细胞的存活率。本研究旨在探讨经Vit E和Se处理的MSC条件培养基(CM)对免疫细胞的影响。方法:用Vit E和Se分离培养MSCs。用Vit E和Se处理的MSC CM培养未成熟树突状细胞(DCs)和外周血单个核细胞(PBMCs)。观察成熟DC上HLA-DR、CD86、CD40、CD83的表达情况。收集DC上清和PBMCs上清，研究TGF-β、IL-10、IL-12。评估PBMCs中T-bet、GATA3、rorr γt和FOXP3的表达。结果:MSC CM增加髓系DC (mDC)的CD40。骨髓间充质干细胞(Vit E)和骨髓间充质干细胞(Se) CM处理的DC细胞CD40降低。在MSC (Vit E) CM中，dc上HLA-DR的表达和IL-12水平显著降低。MSC (Vit E)和MSC (Se) CM处理的dc中IL-10浓度升高。用MSC CM治疗PBMCs可降低IL-10水平、FOXP3和RORγt的表达。另一方面，MSC (Vit E) CM和MSC (Se) CM降低了IL-10水平，升高了IL-12、T-bet和r - γt。结论:结果表明，Vit E和Se处理MSC可增强MSC抑制dc的能力，改善其免疫调节作用。关于间充质干细胞对PBMC的影响，似乎是通过单核细胞增加rorγ - t的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell investigation Biochemistry, Genetics and Molecular Biology-Developmental Biology

CiteScore

5.80

自引率

0.00%

发文量

期刊介绍： The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.