Human Acquired Aplastic Anemia Patients' Bone-Marrow-Derived Mesenchymal Stem Cells Are Not Influenced by Hematopoietic Compartment and Maintain Stemness and Immune Properties.

IF 2.2 Q3 HEMATOLOGY

Anemia Pub Date : 2021-04-29 eCollection Date: 2021-01-01 DOI:10.1155/2021/6678067

Vandana Sharma, Sonali Rawat, Suchi Gupta, Sweta Tamta, Rinkey Sharma, Tulika Seth, Sujata Mohanty

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引用次数: 3

Abstract

Methods: In the current study, we investigated the morphological differences, proliferation capacity, population doubling time (PDT), surface marker profiling, trilineage differentiation potential, and immunosuppressive ability of BM Mesenchymal Stem Cells (BM-MSCs) from untreated aAA patients and in the same number of age- and gender-matched controls.

Results: We observed similar morphology, proliferation capacity, phenotype, trilineage differentiation potential, and immunomodulatory properties of BM-MSCs in aAA patients and control subjects.

Conclusion: Our results confirm that the basic and immunosuppressive properties of BM-MSCs from aAA patients do not differ from normal BM-MSCs. Our data suggest that BM-MSCs from aAA patients might not be involved in disease pathogenesis. However, owing to a smaller number of samples, it is not conclusive, and future studies with more exhaustive investigation at transcriptome level are warranted.

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人获得性再生障碍性贫血患者骨髓间充质干细胞不受造血区室影响并保持干性和免疫特性

方法:在目前的研究中，我们研究了未经治疗的aAA患者和相同数量的年龄和性别匹配的对照组的BM间充质干细胞(BM- mscs)的形态学差异、增殖能力、群体倍增时间(PDT)、表面标记谱、三岁分化潜力和免疫抑制能力。结果:我们观察到aAA患者和对照组BM-MSCs的形态、增殖能力、表型、三期分化潜力和免疫调节特性相似。结论:我们的研究结果证实，aAA患者的骨髓间充质干细胞的基本特性和免疫抑制特性与正常骨髓间充质干细胞没有区别。我们的数据表明来自aAA患者的BM-MSCs可能与疾病的发病机制无关。然而，由于样本数量较少，这并不是决定性的，未来的研究需要在转录组水平上进行更详尽的调查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anemia HEMATOLOGY-

CiteScore

4.80

自引率

3.40%

发文量

审稿时长

18 weeks

期刊介绍： Anemia is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all types of anemia. Articles focusing on patient care, health systems, epidemiology, and animal models will be considered, among other relevant topics. Affecting roughly one third of the world’s population, anemia is a major public health concern. The journal aims to facilitate the exchange of research addressing global health and mortality relating to anemia and associated diseases.