Effects of Xenogen Mesenchymal Stem Cells and Cryo-Platelet Gel on Intractable Wound Healing in Animal Model (Rat).

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2021-01-01 DOI:10.2174/1871523020666210514002722

Babak Alavi-Farzaneh, Ali Shojaeian, Mehdi Banitalebi-Dehkordi, Fatemeh Mirahmadi, Ameneh Mehri-Ghahfarrokhi, Alireza Ghorbanpour, Shima Rahmati-Dehkordi, Farshad Yazdani

{"title":"Effects of Xenogen Mesenchymal Stem Cells and Cryo-Platelet Gel on Intractable Wound Healing in Animal Model (Rat).","authors":"Babak Alavi-Farzaneh, Ali Shojaeian, Mehdi Banitalebi-Dehkordi, Fatemeh Mirahmadi, Ameneh Mehri-Ghahfarrokhi, Alireza Ghorbanpour, Shima Rahmati-Dehkordi, Farshad Yazdani","doi":"10.2174/1871523020666210514002722","DOIUrl":null,"url":null,"abstract":"Background: Today, the effects of growth factors and mesenchymal stem cells (MSCs) in promoting wound healing has been confirmed.Objective: This study aimed to investigate the effect of MSCs and platelet cryogel on wound healing.Methods: 40 male wistar rats were randomly divided into five groups (n=8). The control group was just dressed, the second group received platelet cryogel, the third group received platelet cryogel containing MSCs, the fourth group received plasma, and the fifth group received plasma plus MSCs. The biopsy was obtained from the wounds in the 2, 4, 6, and 8 days of the treatment. Then, pathological evaluation was conducted. Finally, qRT-PCR was performed to determine angiogenesis.Results: The intervention groups had faster wound healing and lower wound area than the control group (p<0.05). The highest wound healing rate and the smallest wound area was observed in the group receiving platelet cryogel plus MSCs. Angiogenesis, fibrosis, myoepithelial and epithelialization in the pathologic examination using H & E staining were not significantly different between the groups. The expression of Ang-1 in the intervention groups was higher than the control group and the highest expression was observed in the platelet cryogel plus MSCs, followed by the platelet cryogel group. The expression of VEGF in the plasma plus MSCs was higher than in the other groups.Conclusion: Further studies require to determine the effects of combined use of platelet cryogel plus MSCs on other types of wound and evaluate mechanisms involved in wound healing like collagenesis and inflammatory factors.","PeriodicalId":35423,"journal":{"name":"Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1871523020666210514002722","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 1

Abstract

Background: Today, the effects of growth factors and mesenchymal stem cells (MSCs) in promoting wound healing has been confirmed.

Objective: This study aimed to investigate the effect of MSCs and platelet cryogel on wound healing.

Methods: 40 male wistar rats were randomly divided into five groups (n=8). The control group was just dressed, the second group received platelet cryogel, the third group received platelet cryogel containing MSCs, the fourth group received plasma, and the fifth group received plasma plus MSCs. The biopsy was obtained from the wounds in the 2, 4, 6, and 8 days of the treatment. Then, pathological evaluation was conducted. Finally, qRT-PCR was performed to determine angiogenesis.

Results: The intervention groups had faster wound healing and lower wound area than the control group (p<0.05). The highest wound healing rate and the smallest wound area was observed in the group receiving platelet cryogel plus MSCs. Angiogenesis, fibrosis, myoepithelial and epithelialization in the pathologic examination using H & E staining were not significantly different between the groups. The expression of Ang-1 in the intervention groups was higher than the control group and the highest expression was observed in the platelet cryogel plus MSCs, followed by the platelet cryogel group. The expression of VEGF in the plasma plus MSCs was higher than in the other groups.

Conclusion: Further studies require to determine the effects of combined use of platelet cryogel plus MSCs on other types of wound and evaluate mechanisms involved in wound healing like collagenesis and inflammatory factors.

查看原文本刊更多论文

异质间充质干细胞和低温血小板凝胶对动物模型(大鼠)难治性创面愈合的影响。

背景:目前，生长因子和间充质干细胞(MSCs)在促进伤口愈合中的作用已经得到证实。目的:探讨骨髓间充质干细胞与血小板冷冻凝胶对创面愈合的影响。方法:40只雄性wistar大鼠随机分为5组(n=8)。对照组刚穿衣，第二组接受血小板冷冻凝胶，第三组接受含MSCs的血小板冷冻凝胶，第四组接受血浆，第五组接受血浆加MSCs。在治疗的第2、4、6和8天从伤口处取活检。然后进行病理评价。最后采用qRT-PCR检测血管生成情况。结果:干预组创面愈合速度快于对照组，创面面积小(p结论:血小板低温凝胶联合MSCs联合使用对其他类型创面的影响有待进一步研究，并评估胶原、炎症因子等参与创面愈合的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.