Bio-Functionalized Chitosan for Bone Tissue Engineering.

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Paola Brun, Annj Zamuner, Chiara Battocchio, Leonardo Cassari, Martina Todesco, Valerio Graziani, Giovanna Iucci, Martina Marsotto, Luca Tortora, Valeria Secchi, Monica Dettin
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引用次数: 27

Abstract

Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supporting cell attachment to the biomaterial through biochemical cues and interaction with membrane receptors. Since the functionalization with bioactive peptides may alter the chemical and physical properties of the biomaterials, in this study we characterized the biological responses of differently functionalized chitosan analogs. Chitosan analogs were produced through the reaction of GRGDSPK (RGD) or FRHRNRKGY (HVP) sequences, both carrying an aldehyde-terminal group, to chitosan. The bio-functionalized polysaccharides, pure or "diluted" with chitosan, were chemically characterized in depth and evaluated for their antimicrobial activities and biocompatibility toward human primary osteoblast cells. The results obtained indicate that the bio-functionalization of chitosan increases human-osteoblast adhesion (p < 0.005) and proliferation (p < 0.005) as compared with chitosan. Overall, the 1:1 mixture of HVP functionalized-chitosan:chitosan is the best compromise between preserving the antibacterial properties of the material and supporting osteoblast differentiation and calcium deposition (p < 0.005 vs. RGD). In conclusion, our results reported that a selected concentration of HVP supported the biomimetic potential of functionalized chitosan better than RGD and preserved the antibacterial properties of chitosan.

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生物功能化壳聚糖用于骨组织工程。
混合生物材料允许改善材料的生物特性,并已成功地用于植入医学应用。具有生物活性肽的材料的共价和选择性功能化通过生物化学线索和与膜受体的相互作用支持细胞附着在生物材料上,为组织工程提供了有利的结果。由于生物活性肽的功能化可以改变生物材料的化学和物理性质,因此本研究表征了不同功能化的壳聚糖类似物的生物学反应。壳聚糖类似物是通过GRGDSPK (RGD)或FRHRNRKGY (HVP)序列与壳聚糖的反应制备的,它们都带有一个醛端基。对纯化或壳聚糖“稀释”的生物功能化多糖进行了深入的化学表征,并对其抗菌活性和对人原代成骨细胞的生物相容性进行了评价。结果表明,与壳聚糖相比,壳聚糖的生物功能化能提高人成骨细胞的粘附性(p < 0.005)和增殖能力(p < 0.005)。总体而言,HVP功能化壳聚糖的1:1混合物:壳聚糖是保持材料抗菌性能和支持成骨细胞分化和钙沉积的最佳折衷(p < 0.005 vs. RGD)。综上所述,我们的研究结果表明,特定浓度的HVP比RGD更能支持功能化壳聚糖的仿生潜能,并保持壳聚糖的抗菌性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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