Dichlorvos Resistance in the House Fly Populations, Musca domestica, of Iranian Cattle Farms.

IF 0.6 4区医学 Q4 PARASITOLOGY

Journal of Arthropod-Borne Diseases Pub Date : 2020-12-31 eCollection Date: 2020-12-01 DOI:10.18502/jad.v14i4.5271

Ebrahim Ahmadi, Jahangir Khajehali

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引用次数: 4

Abstract

Background: Insecticide resistance is one of the most important problems associated with the control of Musca domestica, due to the potential of the rapid development of resistance to different chemical insecticides. The present study was carried out to evaluate dichlorvos resistance in the house fly populations collected from central regions of Iran, Isfahan Province and Chaharmahal and Bakhtiari Province, during 2017 to 2019.

Methods: Bioassays were carried out using a standard topical application method as well as a fumigation method. The Koohrang population (susceptible) with the lowest LD₅₀ values to dichlorvos was chosen to calculate the resistance ratios (RR). Altered sensitivity of acetylcholinesterase (AChE), a target enzyme for dichlorvos, was investigated.

Results: According to the results, very high levels of dichlorvos resistance were observed in the Mobarake population (RR= 80.25-fold by topical application and 33-fold by fumigation bioassay), and Isfahan population (RR= 107.30-fold by topical application and 43-fold by fumigation bioassay) compared to the Koohrang population. Acetylcholinesterase of the Koohrang population was the most sensitive to inhibition by dichlorvos based on the determination of median inhibitory concentration (IC₅₀), but AChE of Mobarake and Isfahan populations were 741.93- and 343.94- fold less sensitive to inhibition.

Conclusion: The insensitivity of AChE was possibly involved in dichlorvos resistance in the house fly populations.

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伊朗养牛场家蝇种群敌敌畏抗性研究。

背景:由于家蝇对不同化学杀虫剂的抗药性可能迅速发展，因此抗药性是家蝇防治的重要问题之一。本研究旨在评估2017 - 2019年在伊朗中部地区、伊斯法罕省以及查哈尔马哈尔和巴赫蒂亚里省采集的家蝇种群对敌敌畏的抗性。方法:采用标准外用法和熏蒸法进行生物测定。选取对敌敌畏LD50值最低的Koohrang易感种群计算抗性比(RR)。研究了敌敌畏靶酶乙酰胆碱酯酶(AChE)的敏感性变化。结果:Mobarake种群和Isfahan种群对敌敌畏的抗性水平均高于Koohrang种群(外用RR= 80.25倍，熏蒸生物测定RR= 33倍)和Isfahan种群(外用RR= 107.30倍，熏蒸生物测定RR= 43倍)。测定中位抑制浓度(IC50)， Koohrang种群的乙酰胆碱酯酶对敌敌畏的抑制最为敏感，而Mobarake和Isfahan种群的AChE对敌敌畏的抑制敏感性分别为741.93和343.94倍。结论:乙酰胆碱酯酶不敏感可能与家蝇种群对敌敌畏的抗性有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

8 weeks

期刊介绍： The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.